FRAP explanation attempt

[alanan84]

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Ok so we talked about FRAP today in my cell phys class and I had this funny smile the whole time. Luckily I didn't get this question on my version of the DAT but for those studying for it, I thought I'd pass this tidbit on. Keep in mind that I am in no way an expert, I'm just passing on what I got from the lecture.

Ok so we were talking about how people proved that cell membranes are fluid and one of the experiments performed was this FRAP technique. Basically what they did was to label all the proteins in the cell membrane so they show up as white dots. Then they take a laser beam and bleach an area of the membrane. That removes the labels in that area so you do not see any white dots (or likewise, proteins). So then they watched the area and eventually what happened is more and more white dots (again, proteins) moved into the bleached area, proving that the membrane was fluid and that proteins were not anchored down, but able to move about the membrane.

That seems simple, and I have no idea what the question on the DAT is about but maybe that helps someone get the concept behind the FRAP technique. I don't know, I just thought I'd pass it on.

 

[Danny289]

Ok so we talked about FRAP today in my cell phys class and I had this funny smile the whole time. Luckily I didn't get this question on my version of the DAT but for those studying for it, I thought I'd pass this tidbit on. Keep in mind that I am in no way an expert, I'm just passing on what I got from the lecture.

Ok so we were talking about how people proved that cell membranes are fluid and one of the experiments performed was this FRAP technique. Basically what they did was to label all the proteins in the cell membrane so they show up as white dots. Then they take a laser beam and bleach an area of the membrane. That removes the labels in that area so you do not see any white dots (or likewise, proteins). So then they watched the area and eventually what happened is more and more white dots (again, proteins) moved into the bleached area, proving that the membrane was fluid and that proteins were not anchored down, but able to move about the membrane.

That seems simple, and I have no idea what the question on the DAT is about but maybe that helps someone get the concept behind the FRAP technique. I don't know, I just thought I'd pass it on.

thanks, just few months late! 😀

 

[heatwheat]

Thanks.

 

[supraman]

Enough for me! Thanks

 

[doc3232]

Concise explanation, thanks.

 

[dentalplan]

doc...you gotta explain to me - why the change from the einstein avatar to the grim reaper .. lol....?

 

[Deontologist]

Damn....just a few hours late...!!

So does that mean smaller lipids would move more rapidly than the larger ones..?? I said that and I think I got that wrong..!!

 

[Aceofspades]

People have explained FRAP a million times on this site. I know what FRAP is, knew what it was way before the DAT, read all the FRAP explanation posts, and still got FRAPPED on the real DAT. All this focus for one question... which by the way is more conceptual than direct.

 

[Isradoc1983]

Damn....just a few hours late...!!

So does that mean smaller lipids would move more rapidly than the larger ones..?? I said that and I think I got that wrong..!!

why would it be wrong? it sounds right... I don't want to discuss the specific of the question, but my friend who took it a month ago choose the same answer.... he told me it was the answer that made the most sense....

 

[Aceofspades]

why would it be wrong? it sounds right... I don't want to discuss the specific of the question, but my friend who took it a month ago choose the same answer.... he told me it was the answer that made the most sense....

You are discussing the specific question, and the answer as well. Why not just post the entire question and answer choices, along with the correct choice while you're at it?

 

[onetoomany]

doc...you gotta explain to me - why the change from the einstein avatar to the grim reaper .. lol....?

i was thinking the same thing!

 

[topdent1]

it doesn't matter how much you study for this question. I foolishly dedicated a couple hours studying for the FRAP question and when I encountered it, I was like WTF! So, save yourself the headache and forget it. Its 1 question which you have 25% chance of getting right anyway.

 

[alanan84]

it doesn't matter how much you study for this question. I foolishly dedicated a couple hours studying for the FRAP question and when I encountered it, I was like WTF! So, save yourself the headache and forget it. Its 1 question which you have 25% chance of getting right anyway.

I agree, there is no point in spending tons of time studying for this one question however, I made this thread so that those that don't have a clue what FRAP is, can quickly read and get an idea SO THAT they can stand a chance IF they get this question. I didn't get the question on my test and I honestly wouldn't recommend spending more time on FRAP than what is in the opening post. If you are good at reasoning things out, and you have a basic understanding of the concept, you MAY be able to decipher the correct answer.