Gel filtration, Bigger = Faster?...

[MCAT guy]

So correct me if I'm wrong here, on a FL I just saw that gel filtration leads to FASTER elution times for LARGER proteins?

carboxymethyl-cellulose ....because the pores in the beads hinder the smaller proteins that pass through them, unlike the larger proteins that bypass the pores in the beads.

can anyone confirm this

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[lorenzomicron]

So correct me if I'm wrong here, on a FL I just saw that gel filtration leads to FASTER elution times for LARGER proteins?



can anyone confirm this

some UC webpage says this: " [FONT=verdana,helvetica,arial]High molecular weight proteins will go down through a column swiftly, while lower molecular weight proteins take more time. This is because the structure of the gel beads within the column excludes molecules that are too big to pass through the bead pores. The sample mix of proteins is added to the top of the column along with a constant flow of buffer solution to keep the proteins moving. As the proteins flow past the beads, the smaller ones are free to enter through the pores. The excluded big molecules flow swiftly around the beads and thus, quickly exit the bottom of the column. The smaller proteins get there eventually, after passing through many pores on many beads.".

 

[loveoforganic]

Size exclusion chromatography uses these special little polymer beads that have incredibly tiny pores. Proteins that are small enough to fit into the pores take long convoluted paths through the column. Large proteins that can't fit into the pores just slide between the gaps between the beads and elute quickly.

 

[lmarina1]

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