- Joined
- Jun 2, 2017
- Messages
- 395
- Reaction score
- 74
I generally am a confident person. I take pride in doing the best possible work I can.
However, I really messed up today in my lab work. I had been doing the ELISA assay for the past couple weeks and it was constantly yielding poor results.
I met with my PI, and he's a nice guy. But he worked with me to dissect why my ELISA was going wrong. When he saw that I was apparently doing the steps right, he asked me to explain to him the reasons behind why the primary and secondary antibody were used.
I stumbled on the names of the specific antibodies, and that's it...the panic started...I could feel my heart beating at a hundred miles a minute, and perspiration started to form on my forehead.
The PI didn't notice, and pressed on with the questions. Deep inside, I knew the answer to the questions, but under pressure, I over-explained the wrong stuff, and under-explained the important things, and came off as an uninformed numb-skull.
It was so embarrassing.
I look at surgeons and physicians, who think under pressure. I've heard that residents get yelled at all the time in surgical residency. How do you survive that and keep going?
I want to learn to be tough...I'm tired of being a wimp 🙁
Ultimately my PI was upset with my lack of comprehension of the assay despite having done it so many times 🙁 🙁
However, I really messed up today in my lab work. I had been doing the ELISA assay for the past couple weeks and it was constantly yielding poor results.
I met with my PI, and he's a nice guy. But he worked with me to dissect why my ELISA was going wrong. When he saw that I was apparently doing the steps right, he asked me to explain to him the reasons behind why the primary and secondary antibody were used.
I stumbled on the names of the specific antibodies, and that's it...the panic started...I could feel my heart beating at a hundred miles a minute, and perspiration started to form on my forehead.
The PI didn't notice, and pressed on with the questions. Deep inside, I knew the answer to the questions, but under pressure, I over-explained the wrong stuff, and under-explained the important things, and came off as an uninformed numb-skull.
It was so embarrassing.
I look at surgeons and physicians, who think under pressure. I've heard that residents get yelled at all the time in surgical residency. How do you survive that and keep going?
I want to learn to be tough...I'm tired of being a wimp 🙁
Ultimately my PI was upset with my lack of comprehension of the assay despite having done it so many times 🙁 🙁