Feb 18, 2013
186
30
Status
Pre-Medical
Is the Ka any different from the Kd in terms of enzyme kinetics?

upload_2015-9-13_21-27-36.png

The question asks if addition of metal ions increases or decreases the amount of CPFX (ligand) bound to BSA (enzyme). Given: the K0 represents the binding constant in the absence of the metal ions.

Shouldn't the K0 > Ka, therefore meaning that the Ka has increased? The answer according to AAMC is that CPFX binding decreased when metal ions were added.

Thanks to anyone who responds.
 

hypericum

7+ Year Member
Oct 19, 2011
68
52
Status
Medical Student (Accepted)
Ka = 1/Kd. I believe the a stands for association, whereas the d stands for dissociation.

Ka would be [bound enzyme] / [enzyme] [ligand] , so if Ka/K0 < 1, that means there's less bound enzyme after metal ions have been added.