I injected 0.5cc pentabarbitol and...

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I'm so glad I'm not doing that anymore. That was the WORST part of doing research.
 
I used to have to decapitate neonatal rats in order to harvest their brain cells as part of my research. That really makes you think, like what if the neurons are still thinking while they are growing and synapsing in my culture dish. During my freshman summer of college, I had a lab job that required me to kill thousands and thousands of frogs. My lab was doing research on tadpoles, and whenver they would metamorphosize, they would lose their usefulness to the lab meaning that I would have to execute the whole lot of them. I still have nightmares about having to kill so many frogs. Late at night, I sometimes can still hear them ribbiting in my sleep. :scared:
 
You do all this research at the expense of MANY animals in order to advance the "medical base of knowledge" so we can have better standard forms of treatment. Yet I go to the surgical center and I see over half the people obese with hypertension and heart disease and think....."No wonder your f****n' knees hurt and you want NSAIDS in order to feel better. Lose weight, your stomach is hanging past your knees! Arrrgh!😡

Sometimes I think these animals die for no reason and sometimes I think that the only thing that comes out of better treatment is so people don't have to reap the negative effects of their destructive habits.😡

A little animal is pissing and sh*ting all over himself because he knows is gonna die and you want me to feel sorry for you.

Death is death is death is death....I think I am temporarily out of service🙁

Heb
 
Originally posted by Darth Vader
That really makes you think, like what if the neurons are still thinking while they are growing and synapsing in my culture dish.
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Sometimes in the lab they do decapitation on the rats without anesthesia, because of the protocol of the experiment. God, how sad....there little head upside down in the sink looking at a bead of water drip down the into the drain...and then darkness. Makes me feel like sh😡 t.....

Heb
 
Originally posted by dstn2bmd
...Yet I go to the surgical center and I see over half the people obese with hypertension and heart disease and think....."No wonder your f****n' knees hurt and you want NSAIDS in order to feel better. Lose weight, your stomach is hanging past your knees! Arrrgh!😡

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As a formerly obese person who once weighed 320 lbs, I hope you tell a patient more then "lose weight, your stomach is hanging past your knees" when he/she comes to you for treatment.
 
Originally posted by aqs200
As a formerly obese person who once weighed 320 lbs, I hope you tell a patient more then "lose weight, your stomach is hanging past your knees" when he/she comes to you for treatment.
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Duh!..........dude get a life and realize someone is having a real f*cking bad day.

Want a COOKIE!
 
Originally posted by dstn2bmd
Duh!..........dude get a life and realize someone is having a real f*cking bad day.

Want a COOKIE!
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Would an ounce of kindness kill you here?
 
Originally posted by drlexygoat
Would an ounce of kindness kill you here?
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Who are you talking about...the poster who has to make a comment when he obviously knows someone is having a moral dilemma.....or me (who really isn't up to being made to feel guilty for expressing my very negative and contradictory thoughts about medicine).

I am expressing my thoughts and feelings (what?...they always have to be positive and idealistic) about situations that I have encountered....I wasn't picking out one single individual. And no, empathy is really not in me right this moment.

Can we get back to talking about how people felt while doing research.....🙁

Heb
 
i'm lucky, i never had to take bio 2 cause i had ap credit, and thats when we do the dissections
 
Originally posted by Darth Vader
I used to have to decapitate neonatal rats in order to harvest their brain cells as part of my research. That really makes you think, like what if the neurons are still thinking while they are growing and synapsing in my culture dish.
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I did the exact same thing!

were you doing electophys on them?
 
As a physio lab instructor, I used to find small animal surgeries fascinating. I've moved on, and I am getting kinda sick and tired of euthanizing small animals on a weekly basis.

Life can be so esoteric at times...

Bleh...
 
It gets even more interesting when after the euthanization you have to remove feces along with several polyps from a rat with colon cancer.
 
We did chicken surgeries for one of my advanced physio classes. It was interesting work, but now I wonder if it was necessary. I can't recall jack $hit from that course. At the end of the labs, we would load the chickens up with pentabarb, and then cut their hearts with scissors to ensure they were dead. Seems like such a waste of life.
 
Originally posted by Qafas
We did chicken surgeries for one of my advanced physio classes. It was interesting work, but now I wonder if it was necessary. I can't recall jack $hit from that course. At the end of the labs, we would load the chickens up with pentabarb, and then cut their hearts with scissors to ensure they were dead. Seems like such a waste of life.
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And the frozen bags containing 50+ chicken wings at the grocery store isn't?

At least you're learning something.
 
"It gets even more interesting when after the euthanization you have to remove feces along with several polyps from a rat with colon cancer."

well isn't that right up your ally SnS
 
Originally posted by Squat n Squeeze
And the frozen bags containing 50+ chicken wings at the grocery store isn't?
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Good call, you ever seen a slaughter house? Seeing someone shoot a cow in the head with a nail gun isnt the most pleasant sight.
 
I for one, am VERY thankful for the research that brings my son the chance to grow up. Prior to 1985, almost ALL children with my son's disorder died by the age of 20. Now due to research (yes on animals) my son has been given the chance to grow up and be a man. I love animals and would never wish to see them suffer....but looking into the face of my dear, precious child and watching him laugh.....and realizing that HE LIVES BECAUSE of research makes it all worth it. If you ever need a reason....just ask and I'll send you a picture of an eleven year old boy with DREAMS of being an engineer WHEN HE GROWS UP!!!!!!
 
Originally posted by CD
I for one, am VERY thankful for the research that brings my son the chance to grow up. Prior to 1985, almost ALL children with my son's disorder died by the age of 20. Now due to research (yes on animals) my son has been given the chance to grow up and be a man. I love animals and would never wish to see them suffer....but looking into the face of my dear, precious child and watching him laugh.....and realizing that HE LIVES BECAUSE of research makes it all worth it. If you ever need a reason....just ask and I'll send you a picture of an eleven year old boy with DREAMS of being an engineer WHEN HE GROWS UP!!!!!!
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w3rd🙂
 
Originally posted by CD
I for one, am VERY thankful for the research that brings my son the chance to grow up. Prior to 1985, almost ALL children with my son's disorder died by the age of 20. Now due to research (yes on animals) my son has been given the chance to grow up and be a man. I love animals and would never wish to see them suffer....but looking into the face of my dear, precious child and watching him laugh.....and realizing that HE LIVES BECAUSE of research makes it all worth it. If you ever need a reason....just ask and I'll send you a picture of an eleven year old boy with DREAMS of being an engineer WHEN HE GROWS UP!!!!!!
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You are making a blanket statement about research. I agree that there is plenty of great research out there, and it is essential for the survival of many people like your son. However, there is plenty of frivolous crap out there as well. Just because one can dream up a project and subject animals to pain and suffering in the name of science doesn't mean that one should.
 
Originally posted by Qafas
You are making a blanket statement about research. I agree that there is plenty of great research out there, and it is essential for the survival of many people like your son. However, there is plenty of frivolous crap out there as well. Just because one can dream up a project and subject animals to pain and suffering in the name of science doesn't mean that one should.
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That's probably why, in order to get approved for animal testing even on simple rats, you have to fill out paperwork probably an inch thick PER protocol. And then to go up higher on the foodchain, that paper work goes up and up and the approval time gets longer and longer.

Heh, I hated sacrificing animals, such a pain to clean up afterwards. But hey, in probably less than 5 minutes (not counting clean up time and perfusion time), using a razor blade and a bone cutter, I can get the brain out of any rat with most of the nerves intact 🙂
 
holy cow.... that is pretty much unnecessary. pirahna's are amazing creatures. anyway, now i'm off to lunch, if i can stomach it.
 
As a vegetarian and a scientist, I often end up in situations where I have a conflict of interest. I can't tell you how many times I've criticized myself for being hipocritical. I refuse to eat meat, yet in vert class I found myself wrist deep in cat intestine.

But perhaps this all depends on whether or not the animal was farmed and killed for disection purposes, or if it had died of natural causes and was donated. In med school the later will hopefully hold true. Surely people are not killed for the sake of providing med school students with cadavers. Although I suppose we'll never know for sure....
 
Originally posted by sgilani
"It gets even more interesting when after the euthanization you have to remove feces along with several polyps from a rat with colon cancer."

well isn't that right up your ally SnS
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Oh God....:laugh:
 
Originally posted by CD
I for one, am VERY thankful for the research that brings my son the chance to grow up. Prior to 1985, almost ALL children with my son's disorder died by the age of 20. Now due to research (yes on animals) my son has been given the chance to grow up and be a man. I love animals and would never wish to see them suffer....but looking into the face of my dear, precious child and watching him laugh.....and realizing that HE LIVES BECAUSE of research makes it all worth it. If you ever need a reason....just ask and I'll send you a picture of an eleven year old boy with DREAMS of being an engineer WHEN HE GROWS UP!!!!!!
Click to expand...

The research I am doing is to provide better treatment for children and that really is what motivates me through the hard stuff. I am so happy for your son🙂. Having two children of my own I don't know what I would do if they had an incurable disorder🙁.

Heb
 
dstn2bmd, just a quick congrats on your acceptance to Wake Forest. It's a great school.
 
Originally posted by Jillianrae
dstn2bmd, just a quick congrats on your acceptance to Wake Forest. It's a great school.
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Hey Jillianrae-

Thanks for the congrats🙂

Heb
 
Originally posted by dstn2bmd
The research I am doing is to provide better treatment for children and that really is what motivates me through the hard stuff. I am so happy for your son🙂. Having two children of my own I don't know what I would do if they had an incurable disorder🙁.

Heb
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Thank you....and I have a hunch I know exactly what you'd do! You would hug them.....and cry, and then......you would begin the search for possible treatments. Somewhere along the way, life would be put in perspective, and you'd discover that nothing else really matters. So take it from me.....take a minute to enjoy LIFE and give those kids a hug!!!!!!
 
i used to do research with mice. first, i killed the mice with carbon dioxide (horrible way to die) and then cut out their heart and aortas. the research involved finding the cause of atherosclerosis. at first, of course, i thought it was so cool to cut out the aortas.

but i had to quit cuz i couldn't handle killing the mice. i actually had nightmares with the mice in it. i'm a vegetarian...i couldn't handle doing this day after day even though i understood the benefits of this research. i can't imagine decapitating live mice, tho i know other labs snapped their necks 🙁
 
One of my biochemistry lab professors was notorious around the research labs when it came to working with mice. They said he used a needle and shoved it in their eyes up to their brain to kill them!
We all thought it was a joke until he brought us to his lab after class and showed us. It was the most nastiest thing I had ever seen - blood gushed all over his glove and you could tell the mouse was in an extreme amount of pain when it died. When asked why he did it that way, he replied, "it's fast, I can kill alot of mice this way, and it's no nonsense."
 
just curious, how do you guys/gals find jobs like this?
 
Originally posted by elvingomez
just curious, how do you guys/gals find jobs like this?
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it took me sometime until i found a lab i wanted to work with. most labs are already full or the profs just don't want to accomodate any undergrads. i looked at my school's website for what the profs were researching on (biology, biomedical science, biochemistry, etc.) and e-mailed them. in the e-mail, i explained my interest to their research (so you should write about their research so it sounds like you sorta know what you're talking about). and if i really really wanted to do research, i went to their labs and spoke to them. it takes time but you'll find something you want to do research on. goodluck
 
Originally posted by dstn2bmd
they just wouldn't die. I then had to decapitate them. God....research can be so depressing sometimes.👎
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Pentabarb really sux and is unreliable. Ketamine Xylazine is a much better and shorter acting. But for euthanasia purpuses you should just use isoflurane.
 
Originally posted by trauma_junky
Pentabarb really sux and is unreliable. Ketamine Xylazine is a much better and shorter acting. But for euthanasia purpuses you should just use isoflurane.
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I know for our lab we didn't use iso because it was too expensive for us to be using to kill rats. We used halothane and pentabarb.
 
I think from now on when they are dead or in a deep sleep then I'll cut through the diaghram in order to make sure that they can't physically breath. I think it will be easier for me to deal with and insure that they don't come to. Oh well...

Heb

P.S. On a lighter note what is the general topic of the research that you guys are/were participating in?

Neoplasticity of the nervous system in response to peripheral nerve injury is the general topic for me.🙂
 
Originally posted by dstn2bmd
I think from now on when they are dead or in a deep sleep then I'll cut through the diaghram in order to make sure that they can't physically breath. I think it will be easier for me to deal with and insure that they don't come to. Oh well...

Heb

P.S. On a lighter note what is the general topic of the research that you guys are/were participating in?

Neoplasticity of the nervous system in response to peripheral nerve injury is the general topic for me.🙂
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I am looking at the amplified pain response of nociceptive neurons in the dental pulp in response to dental injury. We then isolate them and treat with NGF and microarray. Hopefully we will develope a drug to block "chronic pain" syndromes. We are not far off.
 
BTW, do you aspirate the syringe to make sure your not in the intestinal lumen? If it gets in the lumen they'll never go down. Mesenteric absorption is better, but depends on how much intraabdominal adipose is present.

If your in the abdomen already, just disect the descending abdominal aorta or one of its major branches. This form of death is painless.
 
Originally posted by trauma_junky
I am looking at the amplified pain response of nociceptive neurons in the dental pulp in response to dental injury. We then isolate them and treat with NGF and microarray. Hopefully we will develope a drug to block "chronic pain" syndromes. We are not far off.
Click to expand...

SWEEEEET! I love pain studies...
 
It's an ip injection.....do you think severing aorta would be more or less gorry (sp?)?

Heb
 
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