• Funniest Story on the Job Contest Starts Now!

    Contest starts now and ends September 27th. Winner will receive a special user banner and $10 Amazon Gift card!

    JOIN NOW
  • Site Updates Coming Next Week

    Site updates are coming next week on Monday, Wednesday, and Friday. Click the button below to learn more!

    LEARN MORE

IHC Staining Patterns

Napoleon1801

Full Member
15+ Year Member
Jul 29, 2004
550
85
306
Does anyone have a quick and dirty excel spreadsheet for the more common stains and what they're used for/description. I already know about pathologyoutlines and immunoquery. Just wondering if someone had a short list to get some of the basics down.
 

ImmaculateTurd

Full Member
Jul 25, 2009
24
0
0
I don't have one but maybe we can start something here:

Keratins are good for carcinomas:
--AE1/AE3
--Pan-cytokeratin
--Cam5.2
--CK7 and CK20 (good for localizing potential sites of origin)

Smooth muscle markers:
--Smooth muscle actin
--Caldesmon
--Desmin

Vascular markers:
--CD34
--CD31

Hematopoietic markers:
--CD3: T-cells
--CD20: B-cells

Proliferation marker:
--Ki-67/MIB-1

Tumor localization markers (the below are transcription factors so they display nuclear localization):
--TTF-1: thyroid or lung tumors
--CDX-2: GI tumors, especially great for ass cancer
--DPC/SMAD4: expression lost in about 50% pancreatic cancers according to the literature; however, empiric practice observation may vary.

Differentiation markers:
--Neuroendocrine: chromogranin, synaptophysin, CD56 might be controversial but works for small cell carcinomas.
--Thyroid: thyroglobulin
--Medullary carcinoma of thyroid: calcitonin
--Mesothelial: D240, calretinin, WT-1
--Prostate: PrSA, PrAP
--Germ cell markers: Oct3/4, SOX2, AFP (also stains some HCCs), PLAP, c-kit
--GISTs: CD34, c-kit, DOG1
--Serous carcinomas of the GYN tract: p53, WT-1
--Renal cell carcinomas: CD10, RCC antigen
--Sex cord stromal tumors of the ovary: inhibin
--Adrenal cortical tumors: inhibin
 
Last edited:
About the Ads
This thread is more than 12 years old.

Your message may be considered spam for the following reasons:

  1. Your new thread title is very short, and likely is unhelpful.
  2. Your reply is very short and likely does not add anything to the thread.
  3. Your reply is very long and likely does not add anything to the thread.
  4. It is very likely that it does not need any further discussion and thus bumping it serves no purpose.
  5. Your message is mostly quotes or spoilers.
  6. Your reply has occurred very quickly after a previous reply and likely does not add anything to the thread.
  7. This thread is locked.