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I have a question, can double immunostaining be done using chromogens? For instance if you want to stain the same cell with two antibodies. I've only heard it being down using fluorescent antibodies.
I have a question, can double immunostaining be done using chromogens? For instance if you want to stain the same cell with two antibodies. I've only heard it being down using fluorescent antibodies.
But can you actually stain the same cell with two antibodies? For a mix of brown and red. Sort of like flow cytometry. I'm wondering if I can stain plasma cells with CD138 and CD56...
That seems like that would be hard to read...
I think that has to be two different slides... I don't know how well you could read two overlapping cytoplasmic stains..
(if it was a nuclear and cytoplasmic (or membranous and cytoplasmic))
But can you actually stain the same cell with two antibodies? For a mix of brown and red. Sort of like flow cytometry. I'm wondering if I can stain plasma cells with CD138 and CD56...
yeah...its done like everyday. Do some research on it.
If they stain different cellular compartments ie-nuclear vs. membranous, they are easily read by anyone not completely brain dead.
Do you get to bill for 2 88342s if they are on the same slide?
Yeah I was wondering if it is possible too. I know it can work for fluorescence tagged antibodies (red + green = yellow) but haven't seen it yet for chromogen tagged antibodies.
yeah...its done like everyday. Do some research on it.
If they stain different cellular compartments ie-nuclear vs. membranous, they are easily read by anyone not completely brain dead.
It can be done, but visual interpretation can be difficult, depending on the pattern of staining. There are imaging systems available that will acquire a specific wavelength and subtract all others, so you can get an image of each chromagen separately, then combine to see which cells are double-positive.