Is there a way to get the value of [E]T from the lineweaver-plot?

This forum made possible through the generous support of SDN members, donors, and sponsors. Thank you.

laczlacylaci

Full Member
7+ Year Member
Joined
Jun 20, 2016
Messages
264
Reaction score
49
upload_2016-8-25_0-5-52.png

The passage seems to indicate that we are able to get the [E]T from the plot, is that even possible?
The question related to this graph was a M1V1=M2V2 problem to get [E]T. So I am guessing no?
 
A Lineweaver-Burke plot shows the rate data for various substrate concentrations at a specific and given [E]total. Therefore, you can't determine the [E]total just from looking at the graph and usually, the [E]total at which the experiment is performed will be specified in the figure legend (when you're reading a paper).
 
Top