Isocitrate Dehydrogenase

  • Thread starter Thread starter Smokey
  • Start date Start date
Showcase Professionalism with the PREview® Exam
This forum made possible through the generous support of SDN members, donors, and sponsors. Thank you.
S

Smokey

Advertisement - Members don't see this ad
So my biochem professor says that NADH is a negative inhibitor of this Krebs enzyme but not a-ketoglutarate (the product). Wikipedia says that a-keto is also an inhibitor. Any ideas for a "good" source? I am trying google right now.

Thanks!


PS If you clicked on this thread after reading the title, you're a geek.
 
Smokey said:
So my biochem professor says that NADH is a negative inhibitor of this Krebs enzyme but not a-ketoglutarate (the product). Wikipedia says that a-keto is also an inhibitor. Any ideas for a "good" source? I am trying google right now.

Thanks!


PS If you clicked on this thread after reading the title, you're a geek.
Click to expand...

I have no idea, since I took bchm 10 years ago, but here is a reference I've used before:

http://www.rpi.edu/dept/bcbp/molbiochem/MBWeb/mb1/MB1index.html

And, yes, I am most certainly a geek. 😎
 
Thanks, I saw that link but it doesn't have much info about enzyme regulation.
 
I was taught that isocitrate dehydrogenase is inhibited by NADH and can also be inactivated by phosphorylation, which prevents it from binding to isocitrate. I double-checked my textbook and it doesn't say anything about a-ketoglutarate inhibiting it (although in the next step a-ketoglutarate dehydrogenase is inhibited by succinyl-CoA). I think wikipedia might be confused.
 
Smokey said:
So my biochem professor says that NADH is a negative inhibitor of this Krebs enzyme but not a-ketoglutarate (the product). Wikipedia says that a-keto is also an inhibitor. Any ideas for a "good" source? I am trying google right now.

Thanks!


PS If you clicked on this thread after reading the title, you're a geek.
Click to expand...

Tee hee hee - I was just impressed that I knew what you were talking about. My book (Pratt and Cornley) says NADH is the major inhibitor. No inhibitory effects of a-ketogluterate are discussed.
 
Perhaps alpha ketogluturate is not viewed as a product inhibiter because it can be readily interconverted to glutamate in aminotransferase reactions.
 
alpha-KG is not an inhibitor.
 
You must log in or register to reply here.
Top Bottom