in case you meant the difference between kinase and phosphatase which actually have somthing in common,
the difference is kinase attaches a phosphate group while phosphatase removes the phosphate group
these are very important in glucagon formation and regulation
phosphorylases are enzymes that catalyze the addition of a phosphate group from an inorganic phosphate (phosphate+hydrogen) to an acceptor, while a kinase transfers a phosphate group from a donor (usually ATP) to an acceptor. I misread before, I thought it said phosphatase.