Km and Vmax

[ihatebluescrubs]

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Hi guys,

For a single enzyme and it's substrate, would the Km and Vmax always be the same? When would it be different (excluding inhibitors)?

 

[Eastfolding]

I don't understand, Km and Vmax are completely different variables.

 

[gettheleadout]

He's asking would the Vmax and Km remain constant across different reaction conditions.

Well, Vmax would change depending on the concentration of the enzyme.


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[Eastfolding]

Km and Vmax are constants across the reaction.

 

[gettheleadout]

Across a single reaction, yes, but Vmax is just Kcat times the total enzyme concentration, so for a given enzyme/substrate pair Vmax can vary between different reaction conditions.