making own PRP

[bedrock]

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Can those who make their own PRP (don't use a kit), please share how you do it? Both the supplies and the steps?

I'm now doing at least 5 PRP cases per month, and at a kit cost of $250, I figure I could save $1000 each month if I could have my staff make the PRP without a kit?

One thing I am definitely curious about, is if you can achieve a similar platelet concentration? For years I've used accelerated biologics with really solid results.
I'd love to save money by making the PRP without a kit, but not if it significantly affects patient outcomes.

 

[TIVAndy]

Put blood in a 2 tubes. Have MA grab them on left hand and right hand. Have them turn 1500 times per minute for 5 minutes. PRP is ready.

Jokes aside who makes them by themselves?

 

[bedrock]

calling @drusso

 

[runfastnow]

Draw 60 cc whole blood
Transfer to 8 ACD-A anticoag tubes
Spin 1500 rpm x 12 minutes (do not go >2000 RPM)
Remove top plasma/platelet layer + discard blood. Do not let blood mix. Transfer removed plasma/platelets to sterile plain tubes.
Spin 3000 rpm x 15 minues
Discard top ~70% of plasma. Mix/agitate remaining plasma + platelet plug.
Total should be ~8cc.

Depending on how fast you/staff can draw blood and do the transfers it sucks up about 20-30 min of staff time. Can obviously do other stuff while centrifuge spins.

 

[lobelsteve]

Draw 60 cc whole blood
Transfer to 8 ACD-A anticoag tubes
Spin 1500 rpm x 12 minutes (do not go >2000 RPM)
Remove top plasma/platelet layer + discard blood. Do not let blood mix. Transfer removed plasma/platelets to sterile plain tubes.
Spin 3000 rpm x 15 minues
Discard top ~70% of plasma. Mix/agitate remaining plasma + platelet plug.
Total should be ~8cc.

Depending on how fast you/staff can draw blood and do the transfers it sucks up about 20-30 min of staff time. Can obviously do other stuff while centrifuge spins.

Is that a yellow yop tube, Because similar to what my lab does but same speed spin x 10 min with draw off after spin 1.

 

[runfastnow]

Yeah - yellow top tube. Best evidence for highest platelet count in PRP.
EDTA supposedly better for growth factors. Clinical outcomes all about the same.

 

[powermd]

Draw 60 cc whole blood
Transfer to 8 ACD-A anticoag tubes
Spin 1500 rpm x 12 minutes (do not go >2000 RPM)
Remove top plasma/platelet layer + discard blood. Do not let blood mix. Transfer removed plasma/platelets to sterile plain tubes.
Spin 3000 rpm x 15 minues
Discard top ~70% of plasma. Mix/agitate remaining plasma + platelet plug.
Total should be ~8cc.

Depending on how fast you/staff can draw blood and do the transfers it sucks up about 20-30 min of staff time. Can obviously do other stuff while centrifuge spins.

How are you removing the top plasma/platelet layer with no red cells? Stick a needle in there and slurp it off?

 

[runfastnow]

How are you removing the top plasma/platelet layer with no red cells? Stick a needle in there and slurp it off?

Exactly. Multi tube holder a la bench science to hold your tubes.
18 gauge 3.5" into the tube. Draw off as much as you can without disturbing the RBC layer.
Takes a few times to get the feel. Advise holding back pressure on plunger entire time or the vacuum in tube can make things messy.

 

[DrSpine]

I collect the blood and then manually shake for 60 seconds vigorously.

 

[olafa]

are you using a cell counter to check concentration?

 

[drusso]

are you using a cell counter to check concentration?

You should. Dose matters.

 

[powermd]

You should. Dose matters.

Suggestions on reasonably priced cell counters?