Mice research advice

[WhtsThFrequency]

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I'm not sure if this belong here but I wanted to get some advice from vet students who have done animal research and the like....

'll be doing research with MBL/lpr mice (lupus mice) and studying the effects of a highly-processed diet (high glycosylated end product) versus low-processed diet.

I'd like to study the expression of anti-ds DNA autoantibodies and some specific cytokines, and I'm guessing sera and tissue samples are the best way to do it? I've never done an in vivo study before, and I'm not even sure if you need to sacrifice the mice to get enough blood to analyze! If anyone has experience with mice work, especially doing ELISA and things, shoot me a PM and I would definitely appreciate it!!!

 

[sheep girl]

When I did some mice related research for a NIH grant, my PI's always had me collect blood after sacrifice (directly from the heart). For rats, after we sediated them, we got the blood right after decapation.

 

[Electrophile]

PM sent!

 

[projekt]

Antibody blots can often be done with small samples, 2-3 drops, which can be routinely collected from submandibular bleeding. ELISA, apparently, can also be done with these amounts. You might not get quantitative data about cytokines from such small amounts, but you could study one thing through repeated sampling and another after a terminal cardiac collection.

As always, search the literature. (PubMed).

 

[tealamutt]

don't forget about retro-orbital bleeds!