nad+, fad

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Pediateix

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can someone explain what happens to NAD+ and FAD when they gain protons/electrons

in glycolysis it has 2H+ at the end of it, i don't get it or would want further clarification

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Look at the mechanisms of NAD+ and FAD. There are chemical changes that occur that may clear up your confusion. I don't know what protons you're referring to though - FADH2 will lose two protons and two electrons (proton and hydride equivalent) whereas NADH will lose only one proton and two electrons (hydride equivalent). Here's a mechanism showing electron transfer between these agents - a hydride equivalent (proton plus two electrons) is lost from NAPH and given to FAD, which also gains a proton from an acid somewhere. You can imagine the reverse process as well, where FADH2 is deprotonated by a base, causing it to give the hydride back to NADP+.

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