Not sure where else to put this. I'm a med student working in a lab at school. I made a solution following my instructor's directions, but it didn't work. Can someone check the calculations? Trying to produce 1 liter of .25% Trypsin .53 milli-molar EDTA in DMEM. Start with 50mM EDTA, 2.5% 10x Trypsin, and plenty of DMEM. Calculations: X * 50mM = .53 mM *1000mL Solve for X to get X=530/50 = 10.6mL of EDTA 2.5% *Z = .25% * 1000mL Z = .25/2.5 * 1000 = 100mL Trypsin 100 + 10.6 = 111.6 1000 - 111.6 = 888.4mL DMEM Thanks in advance!