- Joined
- Dec 26, 2003
- Messages
- 56
- Reaction score
- 1
Not sure where else to put this.
I'm a med student working in a lab at school. I made a solution following my instructor's directions, but it didn't work. Can someone check the calculations?
Trying to produce 1 liter of .25% Trypsin .53 milli-molar EDTA in DMEM.
Start with 50mM EDTA, 2.5% 10x Trypsin, and plenty of DMEM.
Calculations:
X * 50mM = .53 mM *1000mL
Solve for X to get
X=530/50 = 10.6mL of EDTA
2.5% *Z = .25% * 1000mL
Z = .25/2.5 * 1000 = 100mL Trypsin
100 + 10.6 = 111.6
1000 - 111.6 = 888.4mL DMEM
Thanks in advance!
I'm a med student working in a lab at school. I made a solution following my instructor's directions, but it didn't work. Can someone check the calculations?
Trying to produce 1 liter of .25% Trypsin .53 milli-molar EDTA in DMEM.
Start with 50mM EDTA, 2.5% 10x Trypsin, and plenty of DMEM.
Calculations:
X * 50mM = .53 mM *1000mL
Solve for X to get
X=530/50 = 10.6mL of EDTA
2.5% *Z = .25% * 1000mL
Z = .25/2.5 * 1000 = 100mL Trypsin
100 + 10.6 = 111.6
1000 - 111.6 = 888.4mL DMEM
Thanks in advance!