omg, i left my plasmids in ice bucket overnight

[coralfangs]

have they gone bad already?

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[Jolie South]

are they still cold or are they at room temp?

i used to store my plasmids in a -80 C freezer when I made up my stocks and then in a normal fridge for every day use.

 

[Ebuff]

Haha, depends on what buffer/solution they are in, but they are probably fine. DNA is stable at room temp for quite a while. If they are in water or TE buffer, don't worry about it.

 

[Jolie South]

Haha, depends on what buffer/solution they are in, but they are probably fine. DNA is stable at room temp for quite a while. If they are in water or TE buffer, don't worry about it.

yea, i don't think i ever managed to destroy a plasmid stock so they must be ok at room temp. for extended periods.🙂

 

[coralfangs]

muhahahaha thx

stupid supe kept lecturing me about my data not being too prseentable
i was just gonna show him........ and he knows most of the data color
we spent an hour afterwork coloring different excel cells and stuff, i totally forgot about the plasmids in water

 

[Thewrongstuff]

yeah the plasmids are fine

Rapture has made them adapt to the liquidy environment quite well.

 

[Tropicana100]

you should be fine. plasmids last a while at RT even in water, but better if in Tris or TE. it's just kind of gross if the tubes are floating in melted water. eppendorf tubes can get leaky sometimes. if in doubt, just retransform. it's just an extra day of work.

 

[TheNextMD]

No worries, just toss em up w/ a little hot sauce and they'll taste just fine.

 

[soeagerun2or]

muhahahaha thx

stupid supe kept lecturing me about my data not being too prseentable
i was just gonna show him........ and he knows most of the data color
we spent an hour afterwork coloring different excel cells and stuff, i totally forgot about the plasmids in water

With quality control such as this it is no wonder your data are not too presentable.

Don't get me wrong, techniques vary and protocols are meant to be broken, but when results suffer it is best to stick to the book.

 

[SpartanBlueJay2]

Just be glad it was plasmids and not RNA... I've done that before and RNA is definitely not salvageable.

 

[SeheePolyps]

Hahahaha, I spent a good chunk of yesterday extracting RNA from tissue samples that would have taken months to replace... thank God I decided to do the reverse transcription last night because when I got in this morning, all my little tubes were floating in my bucket! 😱

I was worried too, but I tested the samples and they were a-ok. So hopefully you have nothing to worry about. 🙂