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- Feb 18, 2002
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Hey, I figured the MD/PhD section of this might be able to help with a question I had.
Whenever I have visualized DNA with Ethidium Bromide I just used whatever transilluminator was in the lab. Now I am in the process of buying a lamp for a new lab, and I don't know what wavelength is best because I just took it for granted. I've found the model I want and I'm trying to decide if a 254, 302, or 365 nm bulb is best. Does EtBr excite under all these wavelenghts? If so would you recommend going with the higher wavelenghts to cut down on damage to the DNA?
Thanks
Whenever I have visualized DNA with Ethidium Bromide I just used whatever transilluminator was in the lab. Now I am in the process of buying a lamp for a new lab, and I don't know what wavelength is best because I just took it for granted. I've found the model I want and I'm trying to decide if a 254, 302, or 365 nm bulb is best. Does EtBr excite under all these wavelenghts? If so would you recommend going with the higher wavelenghts to cut down on damage to the DNA?
Thanks