RNA Polymerase

[Sublimation]

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Does RNA polymerase undergo exonuclease? if not how does it proof read, or does it not proof read at all ?

Thanks in advance.

 

[joonkimdds]

I am not sure cuz I forgot everything but isn't RNA polymerase the one that has 2 alpha, 1 beta, 1 beta prime and combine with sigma factor?
I think beta had proofread abiilty but i forgot, i need to go back and check.

 

[jay47]

Yes it does proofread, although I didn't know that before I read this article...

http://mbe.oxfordjournals.org/cgi/content/full/22/6/1444#TBL1

Look in Proofreading and Repair by RNA Polymerases, paragraph 6. The whole article is a discussion on RNA repair and a brief history of why it would be more beneficial to repair.

Joonkim, I think you are referring to DNA polymerase proofreading, because I believe you are correct for DNA.

 

[Sublimation]

Sweet thanks guys.

 

[pbure9]

Yes it does proofread, although I didn't know that before I read this article...

http://mbe.oxfordjournals.org/cgi/content/full/22/6/1444#TBL1

Look in Proofreading and Repair by RNA Polymerases, paragraph 6. The whole article is a discussion on RNA repair and a brief history of why it would be more beneficial to repair.

Joonkim, I think you are referring to DNA polymerase proofreading, because I believe you are correct for DNA.

This is from wiki

Although RNAP does not seem to have the 3'exonuclease activity that characterizes the proofreading activity found in DNA polymerase, there is evidence of that RNAP will halt at mismatched base-pairs and correct it.

And I remember reading in destroyer that it doesnt proofread, but what jay founds seems pretty legit.

 

[nze82]

Does RNA polymerase undergo exonuclease? if not how does it proof read, or does it not proof read at all ?

Thanks in advance.

This is a question in destroyer. RNA polymerase is different from DNA polymerase in that it does NOT have the proof-reading ability.