uwsa1 question

[teehees]

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So this question from UWSA1 isn't making sense to me and I wonder if someone can help me out.

If its making a precursor protein 85kd then why did it stop making it?

Or is it because all the labelled amino acid is used up? It seems like a silly question if thats the case (maybe I'm just being sour though)

 

[dfib slim]

I'd say it got used up.

Sent from my SM-N910P using SDN mobile

 

[InquisitiveDoc]

View attachment 204694

So this question from UWSA1 isn't making sense to me and I wonder if someone can help me out.

If its making a precursor protein 85kd then why did it stop making it?

Or is it because all the labelled amino acid is used up? It seems like a silly question if thats the case (maybe I'm just being sour though)

The way i see it, the original protein formed is 85kD, which is subsequently cleaved to form the final product: 2 proteins of 45kD & 35kD. As time moves on, you get more and more cleavage of that precursor 85kD protein, until finally you have no more 85kD protein and all 35kD & 45kD proteins.

You can further tell with the intensity of the bars. In the beginning, its a large 85kD bar. At the end of the reaction, there are large bars representing more 35kD and 45kD. I hope that makes sense.

 

[catzzz88]

If its making a precursor protein 85kd then why did it stop making it?

Or is it because all the labelled amino acid is used up? It seems like a silly question if thats the case (maybe I'm just being sour though)

Hey, I liked this question too.

So the reason is that when you "radiolabel" something, you label and then wash and that is it. From that point on, any additional protein that is made will not become radiolabeled. The other way that I figured this question out was that often when something is "rapidly degraded by proteases" as in (B), it would be cut up into lots of tiny pieces, not two large pieces.

I agree with you that this question was not explained very well.

Hope this helps!

 

[teehees]

Hey, I liked this question too.

So the reason is that when you "radiolabel" something, you label and then wash and that is it. From that point on, any additional protein that is made will not become radiolabeled. The other way that I figured this question out was that often when something is "rapidly degraded by proteases" as in (B), it would be cut up into lots of tiny pieces, not two large pieces.

I agree with you that this question was not explained very well.

Hope this helps!

Hm yes I guess thats a reasonable way to think about it too

Thanks everyone for your replies!