Western Blot

[Cheeezcake]

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Could someone explain to me western blot and function of primary and secondary antibodies?

 

[docneurol]

Could someone explain to me western blot and function of primary and secondary antibodies?

SNOW
DROP

Western blot = Protein analysis

Primary antibody binds the target protein
Secondary antibody has a fluorescent tag and binds the primary antibody (which is bound to the target protein)

(novowb)