What does PRP activation mean

[mraabs]

Hello,
I have recently learned about PRP. I read that before injecting the prepared PRP we need to activate it.
Now, I am wondering:

• what does PRP activation mean, in other word why PRP needs to be activated?
• Is it possible to activate PRP using a "Calcium Gluconate + Calcium Lactobionate" solution?

I would appreciate it if some one could answer these questions.

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[asmallchild]

Hello,
I have recently learned about PRP. I read that before injecting the prepared PRP we need to activate it.
Now, I am wondering:

• what does PRP activation mean, in other word why PRP needs to be activated?
• Is it possible to activate PRP using a "Calcium Gluconate + Calcium Lactobionate" solution?

I would appreciate it if some one could answer these questions.

From a practical viewpoint, PRP activation increases availability of the desired molecules to the treatment site

From a scientific viewpoint, PRP activation results in degranulation of platelets to release growth factors from α-granules and fibrinogen cleavage to initiate matrix formation

Some people use calcium chloride, I prefer calcium gluconate for PRP activation. I don't know what calcium lactobionate is.

 

[mraabs]

Thanks.

I read in the research papers that CaCl2 and Calcium Gloconate are used for PRP activation. However, here I cannot find any single salt CaCl2 or Calcium Gloconate. They are all in combination with other salts. One such combination is "Calcium Gluconate + Calcium Lactobionate". On the label it is mentioned that Calcium Lactobinate is equivalent to elemental Calcium ( I have attached the label here).

For this reason now I am looking for some other ways to activate PRP ... any suggestions? Can I continue and use (Calcium Gluconate + Calcium Lactobionate)?

 

[asmallchild]

Thanks.

I read in the research papers that CaCl2 and Calcium Gloconate are used for PRP activation. However, here I cannot find any single salt CaCl2 or Calcium Gloconate. They are all in combination with other salts. One such combination is "Calcium Gluconate + Calcium Lactobionate". On the label it is mentioned that Calcium Lactobinate is equivalent to elemental Calcium ( I have attached the label here).

For this reason now I am looking for some other ways to activate PRP ... any suggestions? Can I continue and use (Calcium Gluconate + Calcium Lactobionate)?

I would check with your medical supply company for the closest alternative. As I mentioned, I am unfamiliar with calcium lactobionate so I cannot provide any input on whether or not it would be a suitable agent to activate PRP

Perhaps you can try asking around your community to see what other dermatologists are doing?

 

[mraabs]

I have asked around ... most they do not activate their PRP and some even don't know what does activation means.
Thanks any way

 

[Sophia88]

I'm interested in this as well. I've seen some non derm literature in which they've activated using 10% of CaCl2, 10% of autologous thrombin and combinations of the above two. I've also seen Botropase + 3% CaCl2 in the plastic surgery literature.

I do not understand how the current commercially available PRP kits justify such a high price tag (tubes often $100+ each). I'm sure it is just a matter of time before we start finding much more cost-effective ways to deliver PRP.

 

[asmallchild]

I'm interested in this as well. I've seen some non derm literature in which they've activated using 10% of CaCl2, 10% of autologous thrombin and combinations of the above two. I've also seen Botropase + 3% CaCl2 in the plastic surgery literature.

I do not understand how the current commercially available PRP kits justify such a high price tag (tubes often $100+ each). I'm sure it is just a matter of time before we start finding much more cost-effective ways to deliver PRP.

I don't know about other activating agents as I've only used calcium gluconate in the past (some of my coworkers have also had success with calcium chloride as I believe there has been a recent shortage of the gluconate, I haven't done PRP in a while)

I can comment on the kits. I've found them all to be ripoffs. They certainly make preparation a bit easier but I've had good success with PRP injections using simple cheap items that you can purchase from any lab supply store:

Na Citrate specimen tubes (light blue cap)
Calcium Gluconate 10%
Syringes
Syringe connectors
Long needles for drawing (ideally 1.5in)

Even the centrifuge can be more frugally purchased via lab supply stores or eBay rather than going through the commercial kits