What is the difference between an episome and recombinant DNA?

[navneetdh]

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can someone please clarify?

episome: when a plasmid becomes incorporated into the chromosome

 

[bennijai]

Episomes is any genetic material that can be uptaken naturally by the plasmid, such as genetic materials from viruses. Viruses are able to integrate their genetic material with plasmids to cause a variety of consequences (as shown in lysogenic and lytic cycles).

Recombinant DNA is more controlled and doesn't exist naturally. It's basically genetic manipulation. An example is using restriction enzymes to cut a certain piece of DNA and inserting it into plasmid vectors. Usually we would combine genetic sequences that normally don't exist together.

 

[navneetdh]

Episomes is any genetic material that can be uptaken naturally by the plasmid, such as genetic materials from viruses. Viruses are able to integrate their genetic material with plasmids to cause a variety of consequences (as shown in lysogenic and lytic cycles).
are you sure it is something taken up BY the plasmid? Because in detroyer and in another book they say when a plasmid becomes incorporated INTO the chromose.
Recombinant DNA is more controlled and doesn't exist naturally. It's basically genetic manipulation. An example is using restriction enzymes to cut a certain piece of DNA and inserting it into plasmid vectors. Usually we would combine genetic sequences that normally don't exist together.

I now understand the recombinant DNA fine though. Thanks

 

[bennijai]

What I mean by "naturally by the plasmid" it can be taken up by the plasmid (ie. transformation) or it can be incorporated into the plasmid (ie. transduction). I think conjugation would also be classified as bacteria taking up genetic material. Basically episomes occurs naturally, it doesn't require our direct manipulation.

 

[navneetdh]

cool! Thanks a lot 👍🙂