What protocols/biotech do we need to know, and how in depth should we know it?

[Danny L]

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Maybe the section bank was too in depth, but they had a question about how in Southern Blots you're supposed to look for restriction sites which are usually palindromes.

Too specific or is that the level of detail we should know?

Current list
Biotech:
-Northern, Western, Southern Blots
-PCR
-Plasmids and Vectors
Protocols:
-Mass Spec
-UV Spec
-IR Spec
-Proton NMR

Thanks

 

[Spectar]

Maybe the section bank was too in depth, but they had a question about how in Southern Blots you're supposed to look for restriction sites which are usually palindromes. Too specific or is that the level of detail we should know? Unfortunately I don't work in a biology/biochem lab so I never learned these...

Current list
Biotech:
-Northern, Western, Southern Blots
-PCR
-Plasmids and Vectors
Protocols:
-Mass Spec
-UV Spec
-IR Spec
-Proton NMR

Thanks

+Elisa