Why does it say the anions don't attract the cations?

[mariposas905]

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"The amino acids shown in Figure 1 are primarily polar, uncharged amino acids. As a result, they would not be able to interact with the anion exchange chromatography resin (stationary phase). This type of resin is coated with cations, making it positively charged. It is designed to separate proteins and peptides that are negatively charged. Uncharged proteins, or negatively charged proteins, will not be attracted to the resin."

In this statement, it says the resin is coated with cations, but negatively charged proteins won't be attracted to it? How can this be? I feel like I am missing a concept in understanding anion-exhcnage chromatography.

 

[PlsLetMeIn21]

Everything seems fine until that last sentence. Uncharged proteins obviously are not attracted. If the column is a cationic resin, then the anionic proteins will be attracted.

Where did you get that quote?

 

[mariposas905]

Everything seems fine until that last sentence. Uncharged proteins obviously are not attracted. If the column is a cationic resin, then the anionic proteins will be attracted.

Where did you get that quote?

Yeah, that's the part I don't get. It's from Altius FL 7 C/P Section

 

[Blanky]

That question makes sense to me. Which part is tripping you up?

 

[sunshine859]

I believe there's a typo in the last sentence.. should say that positivity-charged proteins are not attracted to the resin.

 

[mariposas905]

That question makes sense to me. Which part is tripping you up?

The last part...it says anions are not attracted to the cationic resin which didn't make sense to me

 

[Blanky]

When i read it I guess I understood what they are talking about and my brain skipped over the typo. I assume you understand the question if you caught the typo.