I Work as a lab tech in a pharmacology lab at a med school.
Training one of the new tech today to euthanize rabbits. We kill the rabbits then dissect out their aorta for smooth muscle twitch testing with steroids. Well we use barbiturates to euthanize them. We weigh the rabbits then calculate the lethal dose and do 1.2x that dose. Anyway we had a baby rabbit today so it didn't fit in the cage restraints that well (need to restrain them during the injection or else they move during the injection... bad). Yea so anyway this little rabbit was to small for the cage, and 'lo and behold he spazzed and twisted just this new tech was giving the injection
.
Tore the vein open and made a bloody mess (literally) and also destroyed our injection point into the rabbit. About 70% of the solution was injected before it happened, which was pretty close to the LD, so the rabbit became essentially brain dead within 30 seconds... but he was still very much alive and breathing.
The new lab tech (female) had her hands covered in blood and was crying at the rabbit who was half dead, and bleeding the death. I quickly alerted the PI and asked where we should inject the rest of the solution. He said
"too late for that, the rabbit is hemorrhaging blood... begin the dissection."
So with this rabbit still alive, and with the other lab tech pretty much in a state of shock, looks like I was the one to spur of the moment do my first surgery on a live being.
The PI stood back and crossed his arms, I swiftly moved over to the instruments tray and began grabbing scissors, forceps, a scalpel, and anything else I would need. I went back to the rabbit with a fistful of metal and took a second hesitation before diving in and cutting into the pericardium, and then moving on to get a clear view of the heart, still beating. We needed the descending aorta so I tried to nudge the heart to one side with a probe, but since it was beating it kept moving back to where I didn't want it.I finally decide to just move in and use my gloved left hand to hold the heart up... and man that was creepy, trying to hold a beating heart up with 3 fingers so I see the aortic arch. I found it and raised an eyebrow at the fact that arteries nearby were translucent enough that I could see the blood flowing through them. Anyway I took a short breath, lined the scissors and made my cut, unleashing the blood supply into the chest cavity.
I got some paper towel and blotted it up as I felt the heart go limp in my other hand (that was a weird sensation). Once that passed I dissected out the the rest of the resending aorta pretty quickly. By this point a small crowd had gathered in a semi circle around me under news that I was 'operating on a live rabbit' I got a slow applause by some of my co-workers when I finished, but the best was the PI Looked at me and smiled "Nice work, you would make a half decent surgeon"
To bad he already wrote my LOR like 3 months ago
Oh well, it was a pretty exciting experience regardless.