Google is your friend...
Basically, come up with an idea of the gene (simple gel electrophoresis to determine difference between resistant strains and susceptible strains. (and how it works)), design a gene knock out experiment (don't copy the pub I listed, as their techniques are dated). You can be lazy and knock out the gene with insertions of group II introns.
Don't forget... siRNA will not work! Prokaryotes express RNase III
EDIT: If you wanna wow them, tag the protein as suggested, then insert it into the susceptible strain, and test for resistance, thereby proving the gene, gene sequence, locus, and protein. This is theoretical... so its SUPER easy (doesn't have to be realistic or cost effective). "We designed monoclonal ab to the protein and did affinity chromatography, tested purity w/SDS Page..."
EDIT #2: ok, last one then bed. You could link RecA gene into humans (RAD51) and close the question with my favorite one liner of all times "... and this research may someday lead to a cure for cancer"
Finally, don't forget to include an indicator (GFP perhaps?) My work is on Eukaryotes, not pro, so im not too up to date on whats new and hot with them.
The IrrE Protein of Deinococcus radiodurans R1 Is a Novel Regulator of recA Expression
BTW, pretty standard stuff for a Molecular class... which is how many schools are teaching genetics these days
"IRS24 is a DNA damage-sensitive strain of Deinococcus radiodurans strain 302 carrying a mutation in an uncharacterized locus designated irrE. Five overlapping cosmids capable of restoring ionizing radiation resistance to IRS24 were isolated from a genomic library. The ends of each cloned insert were sequenced, and these sequences were used to localize irrE to a 970-bp region on chromosome I of D. radiodurans R1. The irrE gene corresponds to coding sequence DR0167 in the R1 genome. The irrE gene encodes a 35,000-Da protein that has no similarity to any previously characterized peptide. The irrE locus of R1 was also inactivated by transposon mutagenesis, and this strain was sensitive to ionizing radiation, UV light, and mitomycin C. Preliminary findings indicate that IrrE is a novel regulatory protein that stimulates transcription of the recA gene following exposure to ionizing radiation."
