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I thought it's the stuff you get from Starbucks. But thanks for the alternative view. 🙂
 
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This is definitely something you will want to know. Know the general idea is NOT good enough, you have to know the fundamentals and basis very well. It may make a difference in your bio score.
 
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jigabodo said:
This is definitely something you will want to know. Know the general idea is NOT good enough, you have to know the fundamentals and basis very well. It may make a difference in your bio score.
Click to expand...

I'm so anxious to get this question. I've read so much stuff about FRAP/FLIP that I'm hoping that it will help me out a bit in Cell Bio in the future as well.

There just doesn't seem to be THAT much too it, unless you get into the crazy equations and stuff. But I understand the graphs, the way you can measure the binding of proteins...

I was thinking about this though: if you were to have an integral protein and a peripheral protein, is one generally less fluid in the membrane than the other? As in, will one have more lateral movement?

I wouldn't see why one would, unless that protein is maybe used in the support of the cytoskeleton for attachment of microtubules/microfilaments.
 
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If it was attached to microfilaments or stuck in a lipid raft it wouldn't move and or move not so much. I dunno if they would asks you graphs really. If they do then you know that proteins have to move into the spot bleached and if you don't see a protein moving then it is probably stuck on something like you mentioned.
 
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sacjumpman said:
I'm so anxious to get this question. I've read so much stuff about FRAP/FLIP that I'm hoping that it will help me out a bit in Cell Bio in the future as well.

There just doesn't seem to be THAT much too it, unless you get into the crazy equations and stuff. But I understand the graphs, the way you can measure the binding of proteins...

I was thinking about this though: if you were to have an integral protein and a peripheral protein, is one generally less fluid in the membrane than the other? As in, will one have more lateral movement?

I wouldn't see why one would, unless that protein is maybe used in the support of the cytoskeleton for attachment of microtubules/microfilaments.
Click to expand...

Wouldn't you mind to post your findings on FRAP/FLIP for the sake of public benefit?!
 
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Ocean5 said:
Wouldn't you mind to post your findings on FRAP/FLIP for the sake of public benefit?!
Click to expand...

It's nothing really special. Nothing I have saved.

Just Wikipedia and a handful of google results. The normal stuff. The technique itself is relatively basic, IMO. Check out Wiki and you'll see what I mean.

It's applying the ideas that can get tricky.
 
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I'm not very knowlegable with FRAP involving macromolecules other than proteins because that is all I have been exposed to. I'll run this by my molecular cell professor and see how much more info I can get!
 
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TheWiredNerv said:
If it was attached to microfilaments or stuck in a lipid raft it wouldn't move and or move not so much. I dunno if they would asks you graphs really. If they do then you know that proteins have to move into the spot bleached and if you don't see a protein moving then it is probably stuck on something like you mentioned.
Click to expand...

Right, but those were the only proteins that I could think of that would not have much lateral mobility...

I was trying to think of other examples where a protein's lateral mobility would be decreased without much luck.
 
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sacjumpman said:
It's nothing really special. Nothing I have saved.

Just Wikipedia and a handful of google results. The normal stuff. The technique itself is relatively basic, IMO. Check out Wiki and you'll see what I mean.

It's applying the ideas that can get tricky.
Click to expand...


I'm looking at this Graph from Wiki on FRAP, it isn't that expressive, and It doesn't have any explanation. Would you like to say something about the graph?
 
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sacjumpman said:
It's nothing really special. Nothing I have saved.

Just Wikipedia and a handful of google results. The normal stuff. The technique itself is relatively basic, IMO. Check out Wiki and you'll see what I mean.

It's applying the ideas that can get tricky.
Click to expand...

It's true. I took Cell Bio lab which I belive had 1 lecture dedicated to just that technique, but up till this day I still don't know the answer of the FRAP question on DAT...
 
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Ocean5 said:
I'm looking at this Graph from Wiki on FRAP, it isn't that expressive, and It doesn't have any explanation. Would you like to say something about the graph?
Click to expand...

You can try doing a search regarding FRAP on this forum, I am sure some results will pop up. I think there's a thread called last minute review where I tried to explain what it is, but it was quite crappy.

I think I remember how the FRAP graph works, but I wouldn't be able to explain it well because 1. I am kinda hazy on that and 2. you actually need the graph to explain...
 
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jigabodo said:
This is definitely something you will want to know. Know the general idea is NOT good enough, you have to know the fundamentals and basis very well. It may make a difference in your bio score.
Click to expand...

I've read AP cliff for lab stuff, however, it is too basic. I took a molecular bio class, but we did just three experiments overall. Unfortunatly it will efect my score, I know. But do you have any specific source that we could rely on for bio lab application problems. Just some examples of each. B/c I think examples do a better job tha the mere explanation as you mentioned. Camp bio has just one chapter on DNA technology, but nothing on other stuff.
 
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I don't quite know the answer to that question either jigabodo.

sacjumpman, a peripheral membrane protein can become stuck as well depending on if it moves past a hard part of the membrane or a spot that has chemistry preventing it from moving through it. It can also just get stuck on other junk (microfilaments, other proteins, big lipids or fatty acids etc.) and not move.
 
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TheWiredNerv said:
I don't quite know the answer to that question either jigabodo.

sacjumpman, a peripheral membrane protein can become stuck as well depending on if it moves past a hard part of the membrane or a spot that has chemistry preventing it from moving through it. It can also just get stuck on other junk (microfilaments, other proteins, big lipids or fatty acids etc.) and not move.
Click to expand...

Okay, makes sense. Thanks 🙂
 
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so even with all this research and stuff after the exam, you guys still can't figure out the answer to that question? That's utterly ridiculous...
 
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Ocean5 said:
I've read AP cliff for lab stuff, however, it is too basic. I took a molecular bio class, but we did just three experiments overall. Unfortunatly it will efect my score, I know. But do you have any specific source that we could rely on for bio lab application problems. Just some examples of each. B/c I think examples do a better job tha the mere explanation as you mentioned. Camp bio has just one chapter on DNA technology, but nothing on other stuff.
Click to expand...

Don't worry too much about the lab stuff.

Everyone I've spoke to said they had a MAX of 2 lab questions.

And both of the questions, I believe as jigabado can attest, were difficult to the point where they are hard to answer even after studying this stuff in depth.

So don't freak out about it, you know... do your best to understand it, but some of this stuff its tricky to really comprehend how it is applied without have taken a mol bio lab or cell bio class, etc.
 
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craptastic said:
so even with all this research and stuff after the exam, you guys still can't figure out the answer to that question? That's utterly ridiculous...
Click to expand...

That's what is meant by "application" questions. When people say you have to apply the bio info, its tough even with google at your disposal.

Although, I agree, it sucks.
 
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jigabodo said:
You can try doing a search regarding FRAP on this forum, I am sure some results will pop up. I think there's a thread called last minute review where I tried to explain what it is, but it was quite crappy.

I think I remember how the FRAP graph works, but I wouldn't be able to explain it well because 1. I am kinda hazy on that and 2. you actually need the graph to explain...
Click to expand...

Here we go! 😉



FRAP_en.jpg
 
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Ocean5 said:
Here we go! 😉



FRAP_en.jpg
Click to expand...

The graph represents only 1 spot - which is the spot that you originally photobleached. As you can see, initially the fluorescent intensity remains unchanged (flat part), but drops significantly downwards when photobleached. Now, the photobleaching ceases, and you see a rise in fluorescent intensity due to the diffusion of neighboring fluorescent molecules. Now, let me ask you, if the intensity rise back up very quickly, what does it mean in terms of degree of interactions?
 
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that the proteins are more mobile, hence dont have that lipid rift/raft/blockade whatever in their way. theyre basically not attached to anything so can move in rather quickly.
 
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sacjumpman said:
Don't worry too much about the lab stuff.

Everyone I've spoke to said they had a MAX of 2 lab questions.

And both of the questions, I believe as jigabado can attest, were difficult to the point where they are hard to answer even after studying this stuff in depth.

So don't freak out about it, you know... do your best to understand it, but some of this stuff its tricky to really comprehend how it is applied without have taken a mol bio lab or cell bio class, etc.
Click to expand...

I agree. Definitely don't freak out about it. Try to focus on the other bio questions that are more fundamental/basic. Instead of spending 1 hour trying to learn a new technique, it would probably be better to brush up on the things you already know. After all, 2 out of 40 is really not worth it.
 
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jigabodo said:
The graph represents only 1 spot - which is the spot that you originally photobleached. As you can see, initially the fluorescent intensity remains unchanged (flat part), but drops significantly downwards when photobleached. Now, the photobleaching ceases, and you see a rise in fluorescent intensity due to the diffusion of neighboring fluorescent molecules. Now, let me ask you, if the intensity rise back up very quickly, what does it mean in terms of degree of interactions?
Click to expand...
My guess is it means less viscous and more free lipid concentration diffusing around and less protein ratio or the protein did not bind well or some kind of enzyme involvement? What were the choices?
 
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jigabodo said:
The graph represents only 1 spot - which is the spot that you originally photobleached. As you can see, initially the fluorescent intensity remains unchanged (flat part), but drops significantly downwards when photobleached. Now, the photobleaching ceases, and you see a rise in fluorescent intensity due to the diffusion of neighboring fluorescent molecules. Now, let me ask you, if the intensity rise back up very quickly, what does it mean in terms of degree of interactions?
Click to expand...

so what is the answer?
 
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Wow.. I've studied Kaplan, Barrons, and Top Score and haven't seen anything on FRAP.. total freak out.. by the way I am also new on this forum.. is it illegal to ask what type of questions other people have seen on the DAT?
 
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Is there only one FRAP question going around? Or is this FRAP question a TYPE of question.

I'll be disappointed if all this hype is just for one question.
 
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