Biochem Question

Started by dreamer8987
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dreamer8987

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I am having so much trouble with this one question and if anyone could give me any insight that would be great!:
The protease catalyzes the hydrolysis of a heptapeptide substrate with a kcat of 1000 s-1 and a km of 0.075 M. Calculate the Vmax for substrate hydrolysis when HIV-I protease is present at 0.2 mg/ml.
 
dreamer8987 said:
I am having so much trouble with this one question and if anyone could give me any insight that would be great!:
The protease catalyzes the hydrolysis of a heptapeptide substrate with a kcat of 1000 s-1 and a km of 0.075 M. Calculate the Vmax for substrate hydrolysis when HIV-I protease is present at 0.2 mg/ml.
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U have the concentration of the enzyme. u kno that Vmax= kcat [Et] just plug and chug. I am assuming the protease they are talking about is the HIV-I protease. I dont see why they would give u Km. It might be there to throw u off. But u cant use the MM equation cuz ur gonna need Vo at least. Cuz remember u can substitute Vmax with (Kcat[Et]) in that equation.
 
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