Time (s) Product (g)
0 0.0
20 0.25
40 0.50
60 0.70
80 0.80
100 0.85
120 0.85
Q: Why is there no increase in product after 100 sec?
A from the Book: There is no increase because all the enzymes are saturated
and are already catalyzing reactions as fast as they can.
My question: why can't you say that there is no increase because all the substrates have been used up? If there was not enough substrate to react with the enzyme after 100 sec, wouldn't the reaction rate remain constant as well?
please let me know. Thank you in advance!
-----
Additional Question
Q: What would happen if you added only more enzyme after 100 sec?
A from the book: The reaction rate would increase until the enzyme, once again, becomes saturated.
Q: What would happen if you added only more substrate after 100 sec?
A from the book: Nothing
I assumed that there was not enough substrate and an excess amount of enzyme present in above reaction. In this case, wouldn't the reaction proceed and form more products when more substrate is added, while adding more enzymes do nothing?
0 0.0
20 0.25
40 0.50
60 0.70
80 0.80
100 0.85
120 0.85
Q: Why is there no increase in product after 100 sec?
A from the Book: There is no increase because all the enzymes are saturated
and are already catalyzing reactions as fast as they can.
My question: why can't you say that there is no increase because all the substrates have been used up? If there was not enough substrate to react with the enzyme after 100 sec, wouldn't the reaction rate remain constant as well?
please let me know. Thank you in advance!
-----
Additional Question
Q: What would happen if you added only more enzyme after 100 sec?
A from the book: The reaction rate would increase until the enzyme, once again, becomes saturated.
Q: What would happen if you added only more substrate after 100 sec?
A from the book: Nothing
I assumed that there was not enough substrate and an excess amount of enzyme present in above reaction. In this case, wouldn't the reaction proceed and form more products when more substrate is added, while adding more enzymes do nothing?
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