Chances of a question like this...

[JayZee]

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A sample of purified oligopeptide sequence was treated with cyanogen bromide. The original sequence is NH3—Gly-Arg-Phe-Lys-Met-Asp-Tyr-Ala-Arg-Met-Glu—COO— Which of the following is the appropriate cleavage product?

A Gly-Arg-Phe-Lys-Met/Asp-Tyr-Ala-Arg-Met/Glu Cyanogen bromide cleaves peptides on the carboxy-terminus side of methionine. Specific peptide cleavage is important in sequencing.
B Gly-Arg-Phe/Lys-Met-Asp-Tyr/Ala-Arg-Met-Glu Chymotrypsin cleaves peptides on the carboxy-terminus side of the aromatic amino acid residues and leucine.
C Gly-Arg/Phe-Lys/Met-Asp-Tyr-Ala-Arg-Met-Glu Trypsin cleaves peptides on the carboxy-terminus side of arginine and lysine.
D Gly/Arg-Phe-Lys-Met-Asp-Tyr-Ala/Arg-Met-Glu Elastase cleaves peptides on the carboxy-terminus side of the small, uncharged amino acids.
E Gly/Arg-Phe-Lys-Met-Asp-Tyr-Ala-Arg-Met-Glu Aminopeptidases cleave the amino-terminal amino acid residue. These are exopeptidases, which are located in intestinal cells.

I guessed right. TOTALLY GUESSED 👎

 

[mackaikai]

A sample of purified oligopeptide sequence was treated with cyanogen bromide. The original sequence is NH3—Gly-Arg-Phe-Lys-Met-Asp-Tyr-Ala-Arg-Met-Glu—COO— Which of the following is the appropriate cleavage product?

A Gly-Arg-Phe-Lys-Met/Asp-Tyr-Ala-Arg-Met/Glu Cyanogen bromide cleaves peptides on the carboxy-terminus side of methionine. Specific peptide cleavage is important in sequencing.
B Gly-Arg-Phe/Lys-Met-Asp-Tyr/Ala-Arg-Met-Glu Chymotrypsin cleaves peptides on the carboxy-terminus side of the aromatic amino acid residues and leucine.
C Gly-Arg/Phe-Lys/Met-Asp-Tyr-Ala-Arg-Met-Glu Trypsin cleaves peptides on the carboxy-terminus side of arginine and lysine.
D Gly/Arg-Phe-Lys-Met-Asp-Tyr-Ala/Arg-Met-Glu Elastase cleaves peptides on the carboxy-terminus side of the small, uncharged amino acids.
E Gly/Arg-Phe-Lys-Met-Asp-Tyr-Ala-Arg-Met-Glu Aminopeptidases cleave the amino-terminal amino acid residue. These are exopeptidases, which are located in intestinal cells.

I guessed right. TOTALLY GUESSED 👎

well we did learn that in biochem didn't we? but i'm not going to waste my time memorizing where each peptidases cleave the chain...if i see that on the board, i'll just guess like you did and move on =p

 

[JayZee]

If we did, I probably wasn't paying attn.

and I had a year of biochem in college.

HAHAHAHAHAHAHAHAHAH