Haha, I meant oaklandguy; How is too busy studying the effects of kissing butt on the female gender.
Naww, I study a specific immortalized cell line, and I was reading up on some articles about treating brain cancer, and I realized that a few proteins they were inhibiting were forming during a certain disease I am studying and might be causing apoptosis of the cells. So I tried to see if the specific cytokines and kinases would do anything to my cells and I got a dosage dependent result, so I decided to run some gels and I ran a gel with all the proteins found when I injected these kinases, I also ran a zymography and a western blot. On the one gel, I had a couple really really thick lines, so I ran the gel sideways with the whole pH thing, and there were some weird blots, so we digested them and decided we would try to sequence them tomorrow. But as far as my PI knows, he said it didn't look like anything he had seen before. I also found out that the certain proteins that are shown to cause apoptosis in my cell line are still in high concentration; however, the western blot showed them to not be active.
What is making me really happy is that this was all a side project on my own time that I didn't really mention to my PI, so when I showed him the pictures of the cells and all my results he was really excited. I get pretty excited too when I see results; unfortunately it doesn't happen that often and I'm unable to control my emotions because getting sets of data isn't something I'm used to lol.
On the other hand, my main project is supposed to be curcumin's effect on the retina; and it's going miserably. I know it turns on a transcription factor, but I haven't been able to find anything different going on inside the cytoplasm or the nucleus of my cells even with high concentrations of the Indian spice... I'll still play around with it, at least the stuff tastes good too.