DNA repair

[Lindlar]

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Hello
I've been using examkrackers for mcat prep. In the sections about replication, little is said about DNA repair due to mutation and replication errors, however when I glanced through the AAMC mcat guide today I noticed it mentioned these. Does anyone have an idea about the detail of knowledge required here?

All I can immediately recall is that some DNA polymerases have a 3'-5' exonuclease activity for repair during replication, but I remember from molecular cell that there are more complex mechanisms for general mutation... but that seems like a lot of detail for a relatively minor subject.

 

[ARSdogma]

Actually was thinking the exact same thing. EK doesnt cover the repair part thats listed on the AAMC outline, which is funny because they make a big deal about bolding in brown everything that NEEDS to be known on the MCAT. So far, EK seems very simple and straight to the point on everything else though.

 

[Hah]

Just know DNA repair with regards to replication.

DNA Polymerase I - 3-5' and 5-3' exonuclease activity, the latter is more for removing RNA primers in the lagging strand.

DNA Polymerase III- 3-5' exo activity as well

(these two are eukaryotic)

For Prokaryotes there is one DNA pol that has 3-5' exo

 

[Lindlar]

Just know DNA repair with regards to replication.

DNA Polymerase I - 3-5' and 5-3' exonuclease activity, the latter is more for removing RNA primers in the lagging strand.

DNA Polymerase III- 3-5' exo activity as well

(these two are eukaryotic)

For Prokaryotes there is one DNA pol that has 3-5' exo

Forgot about the 5'-3' exonuclease activity, but definitely makes sense with the primers. Prokaryotes not having that ability does raise the question of how they removes the primers from their okazaki fragments though.

 

[ponyo]

Just know DNA repair with regards to replication.

DNA Polymerase I - 3-5' and 5-3' exonuclease activity, the latter is more for removing RNA primers in the lagging strand.

DNA Polymerase III- 3-5' exo activity as well

(these two are eukaryotic)

For Prokaryotes there is one DNA pol that has 3-5' exo

Just in case someone finds this through search like I did and gets horribly misguided-- DNA Polymerases I, II, and III are all prokaryotic... The exo activity listed are correct in that I II III all have 3'-5' exo but I also has 5'-3' for primer removal.