Flow Cytometry

[SBK]

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Would someone mind explaining how to interpret a flow cytometry chart? I've tried googling it but it all links to journal reviews and I just want something basic🙁

 

[21CentSouldier]

Would someone mind explaining how to interpret a flow cytometry chart? I've tried googling it but it all links to journal reviews and I just want something basic🙁

From RR Immuno/Micro, pg 44.

Laser for pew pew pew on cells to determine various factors:
- size
- granularity
- fluorescent properties of cell

1) Light scatter
- front scatter = size of cell
- side scatter = granulations in cell

2) Fluorescent dyes + DNA
- used to eval. cell cycle
- Phases:

- G0/1 = 2n size of fluorescence​

- G2/M = 2x G0/1 fluorescence​

- S = intermediate fluorescence​

3) ImmunoFluorescence
- used to indicate phenotype of cell
- analyze various Abs w/ different colors
- cells identified/quantified via markers on cell

- ie. CD3/CD4, etc...​

- presented as:

- histogram​

- 2-dime dot plot​

 

[ScubaStarved]

http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html

 

[21CentSouldier]

http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html

money 👍