Found a mistake in datQvault

[GeneralChowFun]

---

Members do not see this ad.

I definitely found a mistake in datQvault. Here is the question:

Disulfide bridges are part of which protein structural level?

a. Primary
b. Secondary
c. Tertiary
d. Quaternary
e. More than one of these

I chose "e", than answer is supposed to be "c". All have to say is Antibodies (IgG)

I really hope the DAT test writers don't do the same thing with questions like this, where essentially knowing too much can actually hurt you.

 

[LetsGo2DSchool]

I believe C is correct. Some proteins -- ones that contain cystine amino acids -- make S-S bonds aka disulfide bridges.

 

[HannibalLecter]

Also antibodies are immunoglobulins, globulins = tertiary structure

 

[GeneralChowFun]

I believe C is correct. Some proteins -- ones that contain cystine amino acids -- make S-S bonds aka disulfide bridges.

Agreed, Tertiary Structure is where disulfide bridges CAN first arise. However in some proteins, they may not arise until the quaternary level. This is why I point out IgG, the most easily recognizable form of an antibody. It is composed of 4 sub-units aka a tetramer. Proteins that are organized as more than one sub-unit are considered quaternary.
IgG is composed of two heavy chains and two light chains, the lights chain and heavy chain subunits are linked together by........... disulfide bridges, which do not occur in the tertiary structure of a light chain or a heavy chain but only arise in the quaternary structure of the tetramer.
This is not some esoteric example, but the first thing that popped in my head, i'm sure if I looked around I could find some more examples.... therefore "C" tertiary only is incorrect

If the question said "When can disulfide bridges FIRST arise...." "c' would be correct, but it said "What structure contains disulfide bridges"

 

[GeneralChowFun]

Also antibodies are immunoglobulins, globulins = tertiary structure

Antibodies are tetramers
tetramers = 4 sub units
Sub units = quaternary


..... aren't you the guy that thought a homozygous recessive was a carrier??

 

[FROGGBUSTER]

Yeah it's E. I think insulin is made of more than 1 sub-unit linked together by disulfide bridges.

 

[LetsGo2DSchool]

Good stuff boys. I learned something new.

 

[GeneralChowFun]

Yeah it's E. I think insulin is made of more than 1 sub-unit linked together by disulfide bridges.

So should be worried about this kind of chicanery on the real DAT..... or are they going to have their act together a little bit better....

 

[GeneralChowFun]

Good stuff boys. I learned something new.

👍



Thought I'd make a stink

 

[wired202808]

I would have put down C as well but yes the answer should be more than one. Wiki says that it could also be Tertiary.

Datqvault is user submitted, the company did NOT create these questions; hence errors are possible.

 

[HannibalLecter]

let me school you for a second:

First of all tetramers that you refer to are yes 4 subunits bound to each other. Do you see these subunits in antibodies? No. You see chains. Heavy and light chains at that. You see them in hemoglobin for example, where there are actually 4 different globular subunits. Antibodies don't have subunits.

If you don't believe me look up the definition of an antibody in any biology textbook:

It will say something along the lines of "Antibodies are heavy (~150 kDa) globular plasma proteins."
http://en.wikipedia.org/wiki/Antibody

Globular and fibrous are types of protein's tertiary structure:
"The spherical structure is induced by the protein's tertiary structure."
http://en.wikipedia.org/wiki/Globular_protein

Second, I didn't ask that f*cking question that I stated earlier for your pretentious little views on how you are so good at biology, I asked that question on what the general public thought. If you don't think I know that heterozygous are carriers but homozygous are not you need to see my thread again ***g*t. I asked that question on how the carrier definiton could be altered to include homozygous recessives. Also reconsider the grades you got because obviously all your classmates were dumb as f*ck making you think you actually deserved them in the real world.

 

[GeneralChowFun]

let me school you for a second:

First of all tetramers that you refer to are yes 4 subunits bound to each other. Do you see these subunits in antibodies? No. You see chains. Heavy and light chains at that. You see them in hemoglobin for example, where there are actually 4 different globular subunits. Antibodies don't have subunits.

If you don't believe me look up the definition of an antibody in any biology textbook:

It will say something along the lines of "Antibodies are heavy (~150 kDa) globular plasma proteins."
http://en.wikipedia.org/wiki/Antibody

Globular and fibrous are types of protein's tertiary structure:
"The spherical structure is induced by the protein's tertiary structure."
http://en.wikipedia.org/wiki/Globular_protein

Second, I didn't ask that f*cking question that I stated earlier for your pretentious little views on how you are so good at biology, I asked that question on what the general public thought. If you don't think I know that heterozygous are carriers but homozygous are not you need to see my thread again ***g*t. I asked that question on how the carrier definiton could be altered to include homozygous recessives. Also reconsider the grades you got because obviously all your classmates were dumb as f*ck making you think you actually deserved them in the real world.

I don't need to look up the Mass of IgG, especially not on Wikipedia lol, I've run plenty of SDS-PAGE Gels which show two distinct bands for IgG, this is of course after the molecule is reduced by 2-BME to get rid of those pesky disulfide bonds, the two bands represent polypeptide chains at 25kDa and 50kDa showing that the protein is indeed made up of multiple polypeptide chains/units held together by disulfide bridges.

I was only teasing you, I'm sorry if I offended you, I apologize 😍

 

[GeneralChowFun]

let me school you for a second:

If you don't believe me look up the definition of an antibody in any biology textbook:

It will say something along the lines of "Antibodies are heavy (~150 kDa) globular plasma proteins."
http://en.wikipedia.org/wiki/Antibody

Globular and fibrous are types of protein's tertiary structure:
"The spherical structure is induced by the protein's tertiary structure."
http://en.wikipedia.org/wiki/Globular_protein

Oh and here is a peer reviewed article from the Proceedings of the National Academy of Sciences referring to IgG as having quaternary structure when they two light chains are bound to the two heavy chains via disulfide bridges:
G W Seegan, C A Smith, and V N Schumaker. Changes in quaternary structure of IgG upon reduction of the interheavy-chain disulfide bond. Proc Natl Acad Sci U S A. 1979 February; 76(2): 907–911.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC383089/

Wikipedia is good fun though 👍👍👍👍👍👍👍👍👍👍

 

[HannibalLecter]

Oh and here is a peer reviewed article from the Proceedings of the National Academy of Sciences referring to IgG as having quaternary structure when they two light chains are bound to the two heavy chains via disulfide bridges:
G W Seegan, C A Smith, and V N Schumaker. Changes in quaternary structure of IgG upon reduction of the interheavy-chain disulfide bond. Proc Natl Acad Sci U S A. 1979 February; 76(2): 907–911.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC383089/

Wikipedia is good fun though 👍👍👍👍👍👍👍👍👍👍

Hey man good for you, I was just trying to prove how your logic could be wrong in that initial statement. I didn't want to start any of this but I hope you found your answer.

 

[GeneralChowFun]

Hey man good for you, I was just trying to prove how your logic could be wrong in that initial statement. I didn't want to start any of this but I hope you found your answer.

Well I was out of line bringing up your other thread, I thought it was snarky, but it really was a jerk thing say, my bad dude

 

[toothhornet88]

Well I was out of line bringing up your other thread, I thought it was snarky, but it really was a jerk thing say, my bad dude

lol make up time

 

[indigenoustw]

lol i think for the people on sdn, answer would be c only because that's how most biology books define the characteristics of tertiary structure of proteins when people take intro classes for bio. I'm not saying e is wrong only because I took immuno+lab back in undergrad also so I know what you are talking about. but for the "level" of DAT, i don't think they expect you to know that. It's nice to see such discussions tho, good job.

 

[GeneralChowFun]

lol i think for the people on sdn, answer would be c only because that's how most biology books define the characteristics of tertiary structure of proteins when people take intro classes for bio. I'm not saying e is wrong only because I took immuno+lab back in undergrad also so I know what you are talking about. but for the "level" of DAT, i don't think they expect you to know that. It's nice to see such discussions tho, good job.

Thats what I was afraid of, maybe I have to de-learn some stuff

 

[HannibalLecter]

It's all good dude don't worry about it