GI histo pic plz help

[sadaca]

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hey guys, whats an easy way to distinguish between the layers here in GI?
where are the partial cells found here? They usually ask that or where chief cells. There is also a uw q similar to to this pic, D 124 for those who want to check that out for practice.

I would really appreciate your help

 

[dyspareunia]

I'll type out a more complete answer later, but think about the secretions of both the chief cells and parietal cells. Protein secreting cells have a specific appearance and you can use that to differentiate between the two. Parietal cells are also known for their intracellular secretory canaliculus (when they're actively secreting) and you can use that as well.

As far as the layers of the gastric glands I believe chief cells are in all parts, whereas parietal cells are mainly in the neck/body.

 

[sadaca]

hey thank you, so what would u label as parietal cells here? My main issue is to label them on pics..
thank you!

 

[dyspareunia]

hey thank you, so what would u label as parietal cells here? My main issue is to label them on pics..
thank you!

To me B looks like a parietal (pink plus central nucleus) and C looks like a chief (basal nucleus with apical granules).

 

[sadaca]

hey thanks alot, so parietal cells always look like this, pink with central nucleus? And chief cells have the granules

 

[scoKraz4]

Chief cells always darker b/c they have a ton of RER to make pepsinogen. If the pic is zoomed in enough you should see that the nucleus is located basally and there should be a bunch of granules apically.

The parietal cells will be eosinophilic and contain a ton of mitochondria. They need a lot of mitochondria to maintain the high concentration gradient of H+ inside the lumen of the stomach. I would think that they are pink b/c of the alkaline tide generated from the creation of H+. They will be secreting all the H+ into the stomach (they don't actually have much inside of them) and keep the bicarb in them and secrete it into the blood vessels below them. That is just my theory though regarding the pink staining though.

 

[dyspareunia]

Chief cells always darker b/c they have a ton of RER to make pepsinogen. If the pic is zoomed in enough you should see that the nucleus is located basally and there should be a bunch of granules apically.

The parietal cells will be eosinophilic and contain a ton of mitochondria. They need a lot of mitochondria to maintain the high concentration gradient of H+ inside the lumen of the stomach. I would think that they are pink b/c of the alkaline tide generated from the creation of H+. They will be secreting all the H+ into the stomach (they don't actually have much inside of them) and keep the bicarb in them and secrete it into the blood vessels below them. That is just my theory though regarding the pink staining though.

They're pink because mitochondria are acidophilic.

 

[sadaca]

thanks alot guys really appreciate it