Good "FLAG" antibody

[mark-ER]

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I was hoping one of your labs does a fair deal of immunocytochemistry and you know of a good antibody that would give a clean and strong Immunocytochemistry signal for "FLAG" (the common epitope tag). If it works well with westerns that would be a nice bonus too. Just a catolog # and company works; I plan to order it today or tomorrow. Thanks!!!

 

[Doctor&Geek]

I was hoping one of your labs does a fair deal of immunocytochemistry and you know of a good antibody that would give a clean and strong Immunocytochemistry signal for "FLAG" (the common epitope tag). If it works well with westerns that would be a nice bonus too. Just a catolog # and company works; I plan to order it today or tomorrow. Thanks!!!

We use Anti-FLAG rabbit from Sigma for the purposes of immunofluorescence with reasonably good affinity and background.

 

[Aptamer]

If I recall correctly, the FLAG epitope is patented by Sigma. So, I agree with D&G, use an anti-FLAG (not the band) antibody from Sigma. I like using a biotinylated antibody so that I don't have to use a secondary antibody...just a labelled (strep)avidin for labelling.

F9291 Monoclonal ANTI-FLAG® BioM2 from mouse

Oh yeah, make sure you have the right antibody for the right FLAG tag. Each antibody Sigma offers differing specificities; these specificities are varied positionally i.e. N-terminal, C-terminal, Met-amino-terminal, and internal. The M2 variant does not care about the tags position.

 

[mark-ER]

I wound up getting the M2 Flag antibody from Cell Signaling (they say it's against the same epitope as the one from sigma). Yeah, it's not C- or N- terminal specific and it's cheaper than the sigma (though there's a bit less of it).

I'll re-post about how it works when I run it through>