How do you add an adaptor protein to a treatment?

[ponybreeder4]

---

Members do not see this ad.

This is not homework! I am genuinly curious and cant find an answer anywhere!
So lets say you find some adaptor protein downregulates the expression of some oncogene. How would you include this protein as an agent against the cancer growth. Would you add it to chemotherapy mix?

 

[ILikeDrugs]

why do you need to make multiple threads for each question that you have?

 

[venacontracta]

Are you serious?

Your original thread asking this same question got locked and hasn't even dropped off the first page yet, and you post it again?

 

[tabascosauce]

i dont know what any of those terms mean. could u define them?

 

[venacontracta]

Would you add it to chemotherapy mix?

yea sure just toss a lil bit in

 

[ponybreeder4]

ok can someone please answer this seriously? i have no idea how this process works and im genuinly interested in...can u mix it in or no?

 

[tabascosauce]

ok can someone please answer this seriously? i have no idea how this process works and im genuinly interested in...can u mix it in or no?

you stupid sonofabitch i asked you to help me so i could help u. now you freakin glance over my post like its nothing. youre a real piece of work you know that? i hope you fail at everything in life 👍

 

[LuciusVorenus]

ok can someone please answer this seriously? i have no idea how this process works and im genuinly interested in...can u mix it in or no?

You can mix anything with anything if you just B E L I E V E hard enough.

 

[Iapyx]

you stupid sonofabitch i asked you to help me so i could help u. now you freakin glance over my post like its nothing. youre a real piece of work you know that? i hope you fail at everything in life 👍

u mad?

 

[tabascosauce]

u mad?

nah bra, the fiends be lovin us thugs cause we got the rocks for them pipes

 

[venacontracta]

nah bra, the fiends be lovin us thugs cause we got the rocks for them pipes

yo yo you smoke that adapter protein

 

[ilovelanguages]

I find it odd that most genuine content questions are often locked with a mod saying "no hw allowed". "no content questions allowed" would be a different story. But that would be completely hypocritical as the allopathic forum frequently has threads with medical content. And, is it not their homework to learn how to practice medicine? Hmm...

 

[addo]

i was surprised your other thread got closed, that was some bad modding... this really doesnt sound like a hw questions. well anyways, yea youd probably have to do it by IV, because the protein would get chewed up if you took it orally. But who am i to say, i wouldnt know really.

 

[sedesh]

I'll try and help. You can't introduce the protein into the blood because it won't be able to cross cell membranes and get into the cells, where it needs to be.

So you're probably looking at gene therapy (introducing extragenomic DNA into cells that will make the protein for you). There are loads of problems associated with gene therapy that you can find out about if you do your research.

Long story short, we're not quite there yet.

Read this: http://www.sciencedaily.com/releases/2007/01/070115081330.htm

 

[rHinO1]

sedesh's answer is about as good as you're going to get. There are entire companies, and research labs, that are devoted to improving drug delivery. It is not a simple question.​

If you need a protein to regulate gene expression, then it has to come in contact with DNA. Therefore, the protein has to be intoduced to the nucleus. In order to do that, you must have a mechanism for passing the protein through the cellular membrane, and then the nuclear membrane.​

As sedesh pointed out, it would be more practical to introduce a gene that codes for your "adaptor" protein, and let the cell create the protein. However, intoducing new genes runs the risk of disrupting existing genes. Distruptions include, but are not limited to, causing over/under espression by effecting a promotor, or by causing a truncated or modified protein. The new gene could also convert a proto-oncogene to an oncogene, or the protein could modify the expression of genes besides the one you are interested. In other words, gene therapy is complicated.​

 

[Astarael]

And to follow on top of rHin01's post, you have the problem of what part of the protein creation process you want to attack. If you think back to the central dogma of molecular biology, you have DNA->RNA->protein. If you want to knock out the protein created by an oncogene (which is really the end result you want, since it is the protein that causes problems) you can either disrupt transcription or translation. You've addressed transcription, but there are multiple methods of using RNA interference to keep a protein from being made from the RNA that a cell produces. But then you run into the problem that the oncogene typically has to be mutated to be a problem, and so you won't really know exactly what you have to put into the cell to knock it out. And you still have the problem of how to deliver that therapy into the cell.