making own PRP

[bedrock]

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Can those who make their own PRP (don't use a kit), please share how you do it? Both the supplies and the steps?

I'm now doing at least 5 PRP cases per month, and at a kit cost of $250, I figure I could save $1000 each month if I could have my staff make the PRP without a kit?

One thing I am definitely curious about, is if you can achieve a similar platelet concentration? For years I've used accelerated biologics with really solid results.
I'd love to save money by making the PRP without a kit, but not if it significantly affects patient outcomes.

 

[TIVAndy]

Put blood in a 2 tubes. Have MA grab them on left hand and right hand. Have them turn 1500 times per minute for 5 minutes. PRP is ready.

Jokes aside who makes them by themselves?

 

[bedrock]

calling @drusso

 

[runfastnow]

Draw 60 cc whole blood
Transfer to 8 ACD-A anticoag tubes
Spin 1500 rpm x 12 minutes (do not go >2000 RPM)
Remove top plasma/platelet layer + discard blood. Do not let blood mix. Transfer removed plasma/platelets to sterile plain tubes.
Spin 3000 rpm x 15 minues
Discard top ~70% of plasma. Mix/agitate remaining plasma + platelet plug.
Total should be ~8cc.

Depending on how fast you/staff can draw blood and do the transfers it sucks up about 20-30 min of staff time. Can obviously do other stuff while centrifuge spins.

 

[lobelsteve]

Draw 60 cc whole blood
Transfer to 8 ACD-A anticoag tubes
Spin 1500 rpm x 12 minutes (do not go >2000 RPM)
Remove top plasma/platelet layer + discard blood. Do not let blood mix. Transfer removed plasma/platelets to sterile plain tubes.
Spin 3000 rpm x 15 minues
Discard top ~70% of plasma. Mix/agitate remaining plasma + platelet plug.
Total should be ~8cc.

Depending on how fast you/staff can draw blood and do the transfers it sucks up about 20-30 min of staff time. Can obviously do other stuff while centrifuge spins.

Is that a yellow yop tube, Because similar to what my lab does but same speed spin x 10 min with draw off after spin 1.

 

[runfastnow]

Yeah - yellow top tube. Best evidence for highest platelet count in PRP.
EDTA supposedly better for growth factors. Clinical outcomes all about the same.

 

[powermd]

Draw 60 cc whole blood
Transfer to 8 ACD-A anticoag tubes
Spin 1500 rpm x 12 minutes (do not go >2000 RPM)
Remove top plasma/platelet layer + discard blood. Do not let blood mix. Transfer removed plasma/platelets to sterile plain tubes.
Spin 3000 rpm x 15 minues
Discard top ~70% of plasma. Mix/agitate remaining plasma + platelet plug.
Total should be ~8cc.

Depending on how fast you/staff can draw blood and do the transfers it sucks up about 20-30 min of staff time. Can obviously do other stuff while centrifuge spins.

How are you removing the top plasma/platelet layer with no red cells? Stick a needle in there and slurp it off?

 

[runfastnow]

How are you removing the top plasma/platelet layer with no red cells? Stick a needle in there and slurp it off?

Exactly. Multi tube holder a la bench science to hold your tubes.
18 gauge 3.5" into the tube. Draw off as much as you can without disturbing the RBC layer.
Takes a few times to get the feel. Advise holding back pressure on plunger entire time or the vacuum in tube can make things messy.

 

[DrSpine]

I collect the blood and then manually shake for 60 seconds vigorously.

 

[olafa]

are you using a cell counter to check concentration?

 

[drusso]

are you using a cell counter to check concentration?

You should. Dose matters.

 

[powermd]

You should. Dose matters.

Suggestions on reasonably priced cell counters?

 

[sbp111]

Draw 60 cc whole blood
Transfer to 8 ACD-A anticoag tubes
Spin 1500 rpm x 12 minutes (do not go >2000 RPM)
Remove top plasma/platelet layer + discard blood. Do not let blood mix. Transfer removed plasma/platelets to sterile plain tubes.
Spin 3000 rpm x 15 minues
Discard top ~70% of plasma. Mix/agitate remaining plasma + platelet plug.
Total should be ~8cc.

Depending on how fast you/staff can draw blood and do the transfers it sucks up about 20-30 min of staff time. Can obviously do other stuff while centrifuge spins.

Are you using BD brand yellow top tubes or a tube specifically designed for PRP? Some things I am coming across are saying not to use BD as they are only designed for lab results and not reintroduction into patient and that they may contain endotoxins?

 

[lobelsteve]

Are you using BD brand yellow top tubes or a tube specifically designed for PRP? Some things I am coming across are saying not to use BD as they are only designed for lab results and not reintroduction into patient and that they may contain endotoxins?

And what PRP company do you work for?

 

[sbp111]

And what PRP company do you work for?

I don’t, I’m a pain doc trying to see if I can avoid using expensive kits. I’ve been researching protocols to use vacutainers but have some seen posted mainly on prp company sites like you mentioned describing risks

 

[lobelsteve]

Yellow tops ok.

 

[SpineandWine]

LP-PRP MANUAL EXTRACTION PROTOCOL (DOUBLE-SPIN METHOD)


1. REQUIRED SUPPLIES
- ACD-A anticoagulant tubes
- Tabletop centrifuge (adjustable g-force)
- Sterile transfer pipettes
- 10 mL syringe + 22G needle
- Standard sterile field materials


2. STEP 1 — BLOOD DRAW
- Draw 15–20 mL whole blood into ACD-A tubes.
- Gently invert 5–8× to mix anticoagulant.


3. STEP 2 — FIRST SPIN (SOFT SPIN)
Goal: Separate RBCs without disturbing platelets or WBCs.
- 100–300 g for 5–10 minutes
- Brake OFF
Layering after soft spin:
- Top: Platelet-rich plasma (mostly LP)
- Middle: Buffy coat (WBC layer)
- Bottom: RBCs


4. STEP 3 — PLASMA COLLECTION (CRITICAL FOR LP-PRP)


- Aspirate ONLY the top 2/3 of the plasma layer.
- DO NOT disturb or aspirate the buffy coat.
- Transfer to sterile tube.


5. STEP 4 — SECOND SPIN (HARD SPIN)Goal: Pellet the platelets.


- 400–700 g for 10–17 minutes
- Brake OFF


6. STEP 5 — FINAL LP-PRP PREPARATION


- Discard PPP until 3–5 mL remains.
- Resuspend platelet pellet completely.
- Final product = LP-PRP.


7. QUALITY INDICATORS


- Clear/yellow plasma = LP-PRP.
- Cloudy plasma = leukocyte contamination (LR-PRP).
RECOMMENDED COST-EFFECTIVE SUPPLIES
- BD Vacutainer ACD-A tubes (8.5 mL)
- Eppendorf 5702R centrifuge or LW Scientific C5
- Sterile transfer pipettes
- Standard syringes and needles


LAYER SEPARATION DIAGRAM (ASCII VISUAL)
LP-PRP (ASPIRATE THIS)


------------------------------------


Buffy Coat (DO NOT ASPIRATE)


------------------------------------


RBC Layer


------------------------------------

 

[BobBarker]

I got the Arthrex Angel system. We don’t have time to mess with all of this work with the double spin. So much opportunity for human error.

 

[RoloTomassi]

LP-PRP MANUAL EXTRACTION PROTOCOL (DOUBLE-SPIN METHOD)


1. REQUIRED SUPPLIES
- ACD-A anticoagulant tubes
- Tabletop centrifuge (adjustable g-force)
- Sterile transfer pipettes
- 10 mL syringe + 22G needle
- Standard sterile field materials


2. STEP 1 — BLOOD DRAW
- Draw 15–20 mL whole blood into ACD-A tubes.
- Gently invert 5–8× to mix anticoagulant.


3. STEP 2 — FIRST SPIN (SOFT SPIN)
Goal: Separate RBCs without disturbing platelets or WBCs.
- 100–300 g for 5–10 minutes
- Brake OFF
Layering after soft spin:
- Top: Platelet-rich plasma (mostly LP)
- Middle: Buffy coat (WBC layer)
- Bottom: RBCs


4. STEP 3 — PLASMA COLLECTION (CRITICAL FOR LP-PRP)


- Aspirate ONLY the top 2/3 of the plasma layer.
- DO NOT disturb or aspirate the buffy coat.
- Transfer to sterile tube.


5. STEP 4 — SECOND SPIN (HARD SPIN)Goal: Pellet the platelets.


- 400–700 g for 10–17 minutes
- Brake OFF


6. STEP 5 — FINAL LP-PRP PREPARATION


- Discard PPP until 3–5 mL remains.
- Resuspend platelet pellet completely.
- Final product = LP-PRP.


7. QUALITY INDICATORS


- Clear/yellow plasma = LP-PRP.
- Cloudy plasma = leukocyte contamination (LR-PRP).
RECOMMENDED COST-EFFECTIVE SUPPLIES
- BD Vacutainer ACD-A tubes (8.5 mL)
- Eppendorf 5702R centrifuge or LW Scientific C5
- Sterile transfer pipettes
- Standard syringes and needles


LAYER SEPARATION DIAGRAM (ASCII VISUAL)
LP-PRP (ASPIRATE THIS)


------------------------------------


Buffy Coat (DO NOT ASPIRATE)


------------------------------------


RBC Layer


------------------------------------

That's a pretty low volume blood draw. Even if your platelet % yield is high, which you are already sacrificing by completely avoiding the buffy coat, there's not going to be a ton in only 15 mL whole blood

 

[SpineandWine]

That's a pretty low volume blood draw. Even if your platelet % yield is high, which you are already sacrificing by completely avoiding the buffy coat, there's not going to be a ton in only 15 mL whole blood

I don’t actually do these.
The ortho surgeon texted out his protocol. This is what they had sent out a while back

 

[TIVAndy]

anyone have issues with the PRP part gelatinizing?
i had a girl come we drew blood 3 times and wasted 3 kits due to PRP part gelatinizing

 

[BobBarker]

No adca in the kit?

 

[PinchandBurn]

Are any of you guys concerned about the anticoagulant that is used in here.I know, is a very minimal.Amount, but putting it into the epidural space?

I worry about it even in the commercially available. Kits, or am I just over concerned??

 

[SpineandWine]

Are any of you guys concerned about the anticoagulant that is used in here.I know, is a very minimal.Amount, but putting it into the epidural space?

I worry about it even in the commercially available. Kits, or am I just over concerned??

The clot will start forming inside blood vessel (which is not exposed to anticoagulant)

 

[oreosandsake]

Thought for sure this was a NP doing the procedure

 

[RoloTomassi]

Am I the only one who's never seen this scratching/tapping technique?

 

[lobelsteve]

Am I the only one who's never seen this scratching/tapping technique?

Dr. Jacquelyn Sammons, DO, is a board-certified obstetrician and gynecologist based in Orange County, California. With experience spanning more than a decade, she continues to tend to patients at Transformation Health & Wellness in Anaheim, California.

Dr. Jacquelyn Sammons holds extensive training in the medical field, making her one of the most qualified doctors in her area. She completed her undergraduate degree at California State University, Fullerton. She then earned a Doctor of Osteopathic Medicine from Western University of Health Sciences in Pomona, California. Afterward, Dr. Jacquelyn Sammons went for her internship at the University of California, Los Angeles, before finishing her residency training at the University of California, San Diego. After completion of her academic studies, she returned to Orange County for practice.



She is checking the cervix.

 

[RoloTomassi]

Dr. Jacquelyn Sammons, DO, is a board-certified obstetrician and gynecologist based in Orange County, California. With experience spanning more than a decade, she continues to tend to patients at Transformation Health & Wellness in Anaheim, California.

Dr. Jacquelyn Sammons holds extensive training in the medical field, making her one of the most qualified doctors in her area. She completed her undergraduate degree at California State University, Fullerton. She then earned a Doctor of Osteopathic Medicine from Western University of Health Sciences in Pomona, California. Afterward, Dr. Jacquelyn Sammons went for her internship at the University of California, Los Angeles, before finishing her residency training at the University of California, San Diego. After completion of her academic studies, she returned to Orange County for practice.



She is checking the cervix.

At least she's using a glove unlike your other guy

 

[Ducttape]

her profile pic does not look like her at all.

maybe she should update her profile pic...

 

[oreosandsake]

Am I the only one who's never seen this scratching/tapping technique?

It’s an aesthetics thing
They do it when injecting the face
For sure thought this was a NP

 

[oreosandsake]

It’s an aesthetics thing
They do it when injecting the face
For sure thought this was a NP

Also she used numbing cream all along the lateral knee in the video…. Super weird. Also done in aesthetics. It’s messy cleaning that stuff off. All for a knee injection. BD tubes are not approved to obtain blood products to be injected into people