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My blot worked! In your face guanylate-kinase associated protein! In your face!

Discussion in 'Physician Scientists' started by BNSN, May 7, 2008.

  1. BNSN

    2+ Year Member

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    HAHA.

    I WIN.

    After months.

    MONTHS.

    Of optimizing you.

    Of running failed after failed experiment at differing conditions and getting nothing but a large smear on my membrane.

    I HAVE EMERGED VICTORIOUS.

    I have run you on a test strip.

    And gotten the right bands at the right locations with no background.

    ALL I HAD TO DO WAS HATE MY LIFE FOR 10 MONTHS AND WORK WITH YOU.

    Now, onto you, isoforms 1b/c and 2a/b of various postsynaptic density proteins.

    I will vanquish you too.
     
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  3. Inuranic

    5+ Year Member

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    Science rules.
     
  4. strangeglove

    7+ Year Member

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    Now I know why I'm glad to have a PhD in Neuroscience without ever having run a gel (or a blot, or whatever) in my life. More power to you, though. You'll probably find that things will move a lot faster after this apparent breakthrough.
     
  5. Jorje286

    Jorje286 Member
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    :laugh:

    Most of the stuff I've done so far are non-molecular and I've never done a blot. But I'm definitely not ruling out doing some molecular stuff for the PhD. I know I'm clumsy when it comes to pipetting and some wet-lab techniques, so that post really doesn't bode well for me.
     
  6. M3dhop3ful

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    Man was background your problem? just block for a lot longer than the protocol says..i did it for o/n as opposed to 1 hour and ever since then its worked WONDERS - hardly any background at all
     
  7. QofQuimica

    QofQuimica Seriously, dude, I think you're overreacting....
    Administrator Physician PhD Faculty Lifetime Donor Classifieds Approved 10+ Year Member

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    Just remember this success the next time you feel like your PI is exaggerating about how how well you're doing just to "be nice." And congrats. :)
     
  8. Maxprime

    Maxprime Higgs chaser
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    pwned!
     

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  9. Inuranic

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    Ahaha.

    Epic win.
     

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