While doing UWorld practice, I saw a question that said that a high pI is normally associated with positive charges, and a low pI is associated with negative charges.
The explanation was: if the net charge is (+) at physiological pH, it has a high pI and needs a high pH to deprotonate all of the positively charged side chains.
I never fully understood pI, could someone please explain the above statement as well as a general breakdown on how to understand pH, pI and pKa when asked any of those as an MCAT question?
The explanation was: if the net charge is (+) at physiological pH, it has a high pI and needs a high pH to deprotonate all of the positively charged side chains.
I never fully understood pI, could someone please explain the above statement as well as a general breakdown on how to understand pH, pI and pKa when asked any of those as an MCAT question?