denttiger,
You are correct regarding problem 1. Pretty straight forward.
Ok, problem 2...If I remember correctly cristae are invaginations in the inner membrane of mitochondria. Mesosomes are invaginations in the plasma membrane of prokaryotes. Just a structural analogy question.
The ampicilan resistant gene is used to determine if e.coli were properly transfected. Ampicilan resistant screening will allow selection of transfected E.coli. However, what good is having a plasmid that does not have the gene of interest inserted into the vector? The lacZ gene allows us to determine this. The gene of interest is inserted into the lacZ gene therefore rendering it inactive (we can screen for this to determine if the gene of interest was properly inserted into the vector). Usually, the lacZ gene is partially functional, but this can easily be screened for as well.
Lots of unecessary jargon to distract/intimidate the test taker. This question first exposes your understanding of the difference between a cDNA library and a genomic library. cDNA library only has exonic sequences. Bacteria do not contain splicosomes, therefore they cannot modify a pre-mRNA transcript. Hence, DNA from a cDNA library is desired.
A northern blot allows us to detect RNA and will give us clues about gene expression. Southern blotting is not correct b/c all somatic cells will have the same chromosomal makeup. If the pancreatic cells are expressing insulin, there will be abundant levels of mRNA which can be assayed.
If I recall correctly skeletal muscle cells and nerve cells usually don't undergo mitosis. Therefore, these cells are usually in an "eternal" Go phase. Finding these cells in a G2 phase of the cell cycle is unlikely as this is when the cell prepares to divide.
You are correct, bakers yeast (S. cerevisiae) belongs to ascomycota.
