Random question on immunohistochemistry..

[SweetRain]

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Hi all, I just had a question that needed an answer quickly and couldn't find anyone.

When you stain cells with DAPI and Alexa Fluor and view them under fluorescent microscope, how can you tell if the cells are still viable?

I figured SDN would have the most knowledge and quick with answers lol

 

[bonsaitree1]

Hi all, I just had a question that needed an answer quickly and couldn't find anyone.

When you stain cells with DAPI and Alexa Fluor and view them under fluorescent microscope, how can you tell if the cells are still viable?

I figured SDN would have the most knowledge and quick with answers lol

I believe...to tell if cells are alive, they should still be attached to the flask?

Also..if they're usually rectangular in shape..then if you see that they have become more rounded/circular in shape, then it means they've detached and are dead.

Correct me if I'm wrong.

 

[mcgyver]

live cell membrane is impermeable to DAPI, dead cell membranes are permeable. so if a cell stains dapi, it's dead.

Hi all, I just had a question that needed an answer quickly and couldn't find anyone.

When you stain cells with DAPI and Alexa Fluor and view them under fluorescent microscope, how can you tell if the cells are still viable?

I figured SDN would have the most knowledge and quick with answers lol

 

[mcgyver]

I believe...to tell if cells are alive, they should still be attached to the flask?

Also..if they're usually rectangular in shape..then if you see that they have become more rounded/circular in shape, then it means they've detached and are dead.

Correct me if I'm wrong.

they can be attached and dead. but the ones floating are definitely dead. (given they're adhesive cells). shape really depends on the type of cell you're working on so it's not a good indicator.

 

[JJMrK]

SDN is not the place for homework help.