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The mRNA of a hormone normally produced by an endocrine cell is translated by a free ribosome in the presence and absence of microsomes (derived from the rough ER). In this cell free system, the hormone found to be:
A. the same size in the presence and absence of microsomes
B. longer in the absence of microsomes
C. shorter in the absence of microsomes
D. longer in the presence of microsomes
I think the answer is A. The questions says that with or without a cell present we still get our hormone translated--so I'm thinking that the cell is completely translated in the cytosol and doesn't contain an signal peptide sequence to direct it to the ER for secretion, etc. Therefore, the size would be the same regardless of a cell being present or not. For the record, TBR says the answer is B.
any help would be much appreciated.
A. the same size in the presence and absence of microsomes
B. longer in the absence of microsomes
C. shorter in the absence of microsomes
D. longer in the presence of microsomes
I think the answer is A. The questions says that with or without a cell present we still get our hormone translated--so I'm thinking that the cell is completely translated in the cytosol and doesn't contain an signal peptide sequence to direct it to the ER for secretion, etc. Therefore, the size would be the same regardless of a cell being present or not. For the record, TBR says the answer is B.
any help would be much appreciated.