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What is a good stain for lipofuscin?

Discussion in 'Pathology' started by zxcv1234, Feb 16, 2007.

  1. zxcv1234

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    What is a good stain for lipofuscin?

    Thanks guys!
     
  2. yaah

    yaah Boring
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    H&E

    Seriously.
     
  3. OP
    OP
    zxcv1234

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    thank you sir
     
  4. pathdoc68

    pathdoc68 Member
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    From Silverberg:
    Lipofuscins (lipochrome pigments) are autofluorescent, PAS positive and variably acid fast. They stain with Ziehl-Neelsen. In addition lipofuscin is Sudan black B positive. Lots of pigments look golden/brown and granular on H&E, including melanin, iron and bile pigments.
     
  5. yaah

    yaah Boring
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    Generally, if people want to prove it's lipofuscin, they stain for iron and bile, and maybe melanin if it's really a consideration. But often it just looks like lipofuscin on H&E.
     
  6. PathOne

    PathOne Derminatrix
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    Well, specific options would be HMB-45 staining, or you could try your trusted old Mr. Melanoma-friend, S-100... However, you're probably still likely to be faced with specificity issues.
     
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  7. pathdoc68

    pathdoc68 Member
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    What do you use to stain for bile?
     
  8. georgia_md

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    bile stain or perhaps sudan black
     
  9. pathdawg

    pathdawg Senior Member
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    100% correct. It is usually recognized on h&e. If need be, its diagnosed by exclusion with special stains.
     

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