What is a good stain for lipofuscin?

[zxcv1234]

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What is a good stain for lipofuscin?

Thanks guys!

 

[yaah]

H&E

Seriously.

 

[zxcv1234]

thank you sir

 

[pathdoc68]

From Silverberg:
Lipofuscins (lipochrome pigments) are autofluorescent, PAS positive and variably acid fast. They stain with Ziehl-Neelsen. In addition lipofuscin is Sudan black B positive. Lots of pigments look golden/brown and granular on H&E, including melanin, iron and bile pigments.

 

[yaah]

Generally, if people want to prove it's lipofuscin, they stain for iron and bile, and maybe melanin if it's really a consideration. But often it just looks like lipofuscin on H&E.

 

[PathOne]

Well, specific options would be HMB-45 staining, or you could try your trusted old Mr. Melanoma-friend, S-100... However, you're probably still likely to be faced with specificity issues.

 

[pathdoc68]

Generally, if people want to prove it's lipofuscin, they stain for iron and bile, and maybe melanin if it's really a consideration. But often it just looks like lipofuscin on H&E.

What do you use to stain for bile?

 

[georgia_md]

What do you use to stain for bile?

bile stain or perhaps sudan black

 

[pathdawg]

Generally, if people want to prove it's lipofuscin, they stain for iron and bile, and maybe melanin if it's really a consideration. But often it just looks like lipofuscin on H&E.

100% correct. It is usually recognized on h&e. If need be, its diagnosed by exclusion with special stains.