What is a good stain for lipofuscin?

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pathdoc68

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From Silverberg:
Lipofuscins (lipochrome pigments) are autofluorescent, PAS positive and variably acid fast. They stain with Ziehl-Neelsen. In addition lipofuscin is Sudan black B positive. Lots of pigments look golden/brown and granular on H&E, including melanin, iron and bile pigments.
 

yaah

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Generally, if people want to prove it's lipofuscin, they stain for iron and bile, and maybe melanin if it's really a consideration. But often it just looks like lipofuscin on H&E.
 

PathOne

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Well, specific options would be HMB-45 staining, or you could try your trusted old Mr. Melanoma-friend, S-100... However, you're probably still likely to be faced with specificity issues.
 

pathdoc68

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Generally, if people want to prove it's lipofuscin, they stain for iron and bile, and maybe melanin if it's really a consideration. But often it just looks like lipofuscin on H&E.

What do you use to stain for bile?
 

pathdawg

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Generally, if people want to prove it's lipofuscin, they stain for iron and bile, and maybe melanin if it's really a consideration. But often it just looks like lipofuscin on H&E.

100% correct. It is usually recognized on h&e. If need be, its diagnosed by exclusion with special stains.
 
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