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Discussion in 'Pathology' started by zxcv1234, Feb 16, 2007.
What is a good stain for lipofuscin?
thank you sir
Lipofuscins (lipochrome pigments) are autofluorescent, PAS positive and variably acid fast. They stain with Ziehl-Neelsen. In addition lipofuscin is Sudan black B positive. Lots of pigments look golden/brown and granular on H&E, including melanin, iron and bile pigments.
Generally, if people want to prove it's lipofuscin, they stain for iron and bile, and maybe melanin if it's really a consideration. But often it just looks like lipofuscin on H&E.
Well, specific options would be HMB-45 staining, or you could try your trusted old Mr. Melanoma-friend, S-100... However, you're probably still likely to be faced with specificity issues.
What do you use to stain for bile?
bile stain or perhaps sudan black
100% correct. It is usually recognized on h&e. If need be, its diagnosed by exclusion with special stains.