Xylene substitutes Histology

[BartH]

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Hello,

I really don't know if I came to the right place for this, but I will try.

For a study project I am looking on how to use an alternative for xylene for tissue clearing and staining on our histology lab.

There are tons of substitutes out there, but I was wondering if someone can tell me the difference between

• Aliphatic hydorcarbons
• Limonene based agents

What is the difference and what are the advantages of both compared to xylene.

I hope someone can help me find some information on this subject.