3T3 vs. Caco-2

[HowAboutDAT]

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This post is totally unrelated to DAT material. It's actually a question I have from cell biology lab. Does anyone have any guesses at why a Caco-2 (cancer cell) line would show more apoptosis than 3T3 mouse fibroblast cells when they are deprived of serum?

 

[sciencegod]

maybe becaus it dies more. hope that helps.

 

[HowAboutDAT]

maybe becaus it dies more. hope that helps.

thats not cool homie

 

[bigstix808]

This post is totally unrelated to DAT material. It's actually a question I have from cell biology lab. Does anyone have any guesses at why a Caco-2 (cancer cell) line would show more apoptosis than 3T3 mouse fibroblast cells when they are deprived of serum?

i personally have never done research with these cell lines, but do remember talking about them breifly in bio of cancer...

if i had to guess, i would say that the caco 2 cells are simply more vascular than that of the 3t3 cells (epithelial cells are more vascular than most fibroblasts anyway)...thus their could be a higher dependency on the serum which could trigger the elevated apoptitic trends you are asking about.

what is this info for anyway??

 

[HowAboutDAT]

i personally have never done research with these cell lines, but do remember talking about them breifly in bio of cancer...

if i had to guess, i would say that the caco 2 cells are simply more vascular than that of the 3t3 cells (epithelial cells are more vascular than most fibroblasts anyway)...thus their could be a higher dependency on the serum which could trigger the elevated apoptitic trends you are asking about.

what is this info for anyway??

it was just a question that I had from the cell biology lab course I am taking, totally out of the scope of the DAT (hopefully? haha)

 

[khakwanit1]

Cancerous cells are continuously divided which triggers apoptosis to occur at a higher rate unless there is a mutation. In over 90% of the cancers, p53 is mutated. Lack of serum will cause apoptosis in both the cells and from what I remember, cancer cells can survive for a while without serum/growth factors. I would have to agree with bigstix.

 

[yuppers]

maybe becaus it dies more. hope that helps.

sciencegod..... really

 

[vhlam]

Cancerous cells are continuously divided which triggers apoptosis to occur at a higher rate unless there is a mutation. In over 90% of the cancers, p53 is mutated. Lack of serum will cause apoptosis in both the cells and from what I remember, cancer cells can survive for a while without serum/growth factors. I would have to agree with bigstix.

i don' think you worded your first sentence correctly. apoptosis occurs when something in the cell goes wrong, but cancer cells don't obey these signals, hence, they keep on dividing. therefore, apoptosis rate is very low in cancer cells.

 

[achilles2]

This post is totally unrelated to DAT material. It's actually a question I have from cell biology lab. Does anyone have any guesses at why a Caco-2 (cancer cell) line would show more apoptosis than 3T3 mouse fibroblast cells when they are deprived of serum?

The mutation in the 3T3 fibroblast cells is most likely effecting the P53 pathway, thus they tend to not undergo apoptosis but rather hold out til they perish due to necrosis.

The Caco-2 cell lines have mutations in areas other then the p53 pathway thus they still exp apoptosis. Most likely oncogene mutation that causes over proliferation.


Thats my best guess

i don' think you worded your first sentence correctly. apoptosis occurs when something in the cell goes wrong, but cancer cells don't obey these signals, hence, they keep on dividing. therefore, apoptosis rate is very low in cancer cells.

not all cancer is the same


basically its a tug of war [apoptosis-----------------------------------oncogenes]

if you decrease the aopotosis you net more cell proliferation
on the other hand you could activate oncogenes to increase cell proliferation and the cells could obey apoptosis as normal but the net effect is proliferation

it really depends on where the cells endured the mutation

 

[khakwanit1]

i don' think you worded your first sentence correctly. apoptosis occurs when something in the cell goes wrong, but cancer cells don't obey these signals, hence, they keep on dividing. therefore, apoptosis rate is very low in cancer cells.

You mean those cancer cells in which p53 is mutated, apoptosis occurs at a slow rate. Apoptosis is a natural event that happens in every organism. It doesn't only happen when something goes wrong. Let me give you an example to prove my point. During the development of an offspring of some organism, the fingers are not completely formed and shaped like web. Apoptosis occurs in those areas to form fingers. I do agree that in most cancers p53 is mutated, which prevents it from performing its task.