bio lab techniques

[flycd05]

---

Members do not see this ad.

Does the DAT ask for bio lab techniques (other than FRAP)? i'm not even gonna attempt to learn the FRAP question cuz i've heard its a complete waste of time for 1 question so ill take my chances on guessing for that. My test is on thursday and i've neglected studying the lab techniques like FRAP, ELISA, PCR, X-RAY diffraction, electrophoresis etc i only know the basics that i learned from destroyer but thats it. Are they covered on the DAT like is there usually more than 1 question on it?

 

[Ferdowsi]

to add to your question, so many people here say that there are application questions..what do you mean by application questionS?

 

[tRNA]

what's ELISA, I have never seen that in destroyer, is it this

"blood test used to detect Helicobacter pylori bacteria. Also used to diagnose an ulcer."???

 

[klpennin]

ELISA is Enzyme Linked Immunoflurescent Assay

 

[Simiam]

ELISA is Enzyme Linked Immunoflurescent Assay

Enzyme Linked ImmunoSorbent Assay... ??


The ELISA relies on antibodies to detect the presence of antigens in liquid samples.

 

[topdent1]

Perhaps this would be a good time to introduce any genchem lab techniques?

 

[Simiam]

Electrophoresis is a method to seperate molecules in a mixture by passing them through a gel driven by electric current. The gel is usually placed in an ionic buffer liquid. When the current is applied negatively charged molecules will travel towards the positive node. The farther down the gel they make it the smaller they are (IE the faster they travel). Large molecules travel slower and will be near the top. You usually use ethidium bromide as a flourescent...

 

[Simiam]

PCR is Polymerase Chain Reaction. It allows you to produce large amounts of DNA in a test tube (in vitro) starting from small amounts.

The steps are Denature --> Anneal primers --> Extend chain repeated usually 40x...

 

[flycd05]

wow thanks guys! do we need to know the type of experiments done - like hershey/chase, avery-macleod, miller-urey??

 

[Mstoothlady2012]

wow thanks guys! do we need to know the type of experiments done - like hershey/chase, avery-macleod, miller-urey??

didn't show up on my test. But i would say yes they are important to know. How did they figure out that DNA replication is semiconservative and not conservative?

Also application means applying the knowledge of definition, experiments etc. Basically DAT is not gonna ask you what is the definition of capacitance? Instead it will lay out a scenario for you and you should be able to figure out that they are talking about capacitance and not fertilization or induction or something else. I think DAT questions has a lot of scenarios, atleast in my case I had ~50% of scenario questions. But if you know your material well they are really easy!!!