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Ok this is my last resort I have emailed my TA on this question and he will not respond to me so i figured I would ask yall and see if anyone knows how to do this question. I am not looking for an answer but more for a push in the right direction and I cant find anything on the web about this. Okay here is the question
Consider the following kinetic parameter for a given enzyme
Km=4.7x10-5 M
Vmax= 22 nmol/min/mg
=5x10-1 mM
=2x10-4 M
Ki=3x10-4 M
Calculate the rate of product formation in the presence of a competitive inhibitor.
I know the equation I have to do, and that I have to solve for Vo. I also know that I need to convert the concentrations so that they are all alike so they will work in the equation, but the Vmax concentration he gave me is confusing. On every problem we did he gave us moles/(volume)/(time) which is what it should be, and in this problem it is almost like he is giving the me the specific activity of the enzyme. How would i convert Vmax into something useable for the equation for inhibitors?
any help would be appreciated
Consider the following kinetic parameter for a given enzyme
Km=4.7x10-5 M
Vmax= 22 nmol/min/mg
=5x10-1 mM
Ki=3x10-4 M
Calculate the rate of product formation in the presence of a competitive inhibitor.
I know the equation I have to do, and that I have to solve for Vo. I also know that I need to convert the concentrations so that they are all alike so they will work in the equation, but the Vmax concentration he gave me is confusing. On every problem we did he gave us moles/(volume)/(time) which is what it should be, and in this problem it is almost like he is giving the me the specific activity of the enzyme. How would i convert Vmax into something useable for the equation for inhibitors?
any help would be appreciated
