biology: Genetic technology

[b0b0]

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How vital is it to understand genetic technology like blotting, and stuff like PCR, RFLPs.

 

[ktran17]

Just know the bare basics

here are some basic lab techniques you should know.
PCR:
Western blot:
Southern Blot:
Northern blot:
SDS PAGE
DNA microarray technology
Gram staining

here are some more advance biochem techniques you might not need to know
bradford assay
ELIZA
benedicts test

 

[aqz]

I'd know these:

PCR - DNA multiplication (know all the denaturing, annealing steps).

Gel electrophoresis - Separates by size, attracts nucleic acids down an agarose gel with positive current.

Size exclusion chromatography - Separates by size, larger molecules travel faster than smaller.

DNA Microarray - Measures different gene expression levels simultaneously. Uses "probes".

For blotting: Memorize SNOW DROP

Southern - DNA
Northern - RNA
O O
Western - Protein

 

[412smb412]

lol SNOWDROP! love it! do u have any more useful mnemonics????

 

[magriva]

chad's videos are really helpful for this topic

 

[aqz]

I have a whole santa bag full of mnemonics, but most of them aren't going to make sense like SNOWDROP.

A good one to know is SEVENUP

Seminiferous Tubes
Epididymus
Vas Deferens
Ejaculatory
Nothing (just a placeholder)
Urethra
Penis

"Large Random M & M" for Hardy-Weinberg:

Large Population
Random mating
No mutation
No natural selection
No migrations

You probably already know FLATPEG but if not...these are the anterior pituitary hormones:

FSH
LH
ACTH
TSH
Prolactin
Endorphins
GH

Probably also know this one for classification:

Kings Play Cards On Fat Green Stools.

Nobody Likes My Educational Background (in order of decreasing numbers)

Neutrophil, Lymphocytes, Macrophages, Eosinophil, Basophil

PAN CAME

Pseudocoelomate = arthopod, nematoda
Coelomate = annelida, molluska, echinoderm
Platyhelminths = Acoelomate

I have some alphabetical ones such as...

T comes after C, so stuff enters the Golgi through the Cis face, leaves in secretory vesicles from the Trans face.

Some numerical ones such as.....

700 is bigger than 680, so P700 is photosystem 1 and P680 is photosystem 2

Random ones:

An Ox Red Cat (anode cathode)

in the Basement Spins Grandma Luci in her Corn bowl (think this was from destroyer, for lowest to highest Stratum layers)

That's all for now....my head hurts.

 

[ktran17]

Can I keep selling sex for my money officer ?

Citrate -> Isocitrate-> alpha-ketoglutarate-> succinyl coa-> succinate-> fumarate-> malate-> oxoacetate

also succinate comes after succinyl coa because its always LATE 😀

TCA intermediates ^

 

[b0b0]

Thank you very much for all the help !!!