Frap Question

[wkawk2416]

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What is the % recovery and lateral mobility for large multi-subunit integral membrane protein that is not anchored to the cytoskeleton?

a. High % recovery with fast mobility
b. Low % recovery with fast mobility
c. High % recovery with slow mobility
d. Low % recovery with low mobility

Originally posted by someone, but found in my word document.

 

[osimsDDS]

High percent recovery, but at low mobility or high is the question...

If its not anchored that means it will move throughout the membrane, FRAP = flourensence recovery after photobleaching...so if its moving the laser will catch it at high percent recovery...just i have no idea if its low mobility or high...someone HELP!!!!

 

[wkawk2416]

i think answer is A.
protein that is not anchored to the cytoskeleton wil have high % of recovery which means fast motility.

 

[Thundercatz]

I would guess its A, as it isnt anchored and proteins are free to move laterally.

 

[osimsDDS]

yea its A, its got to be....more mobility = faster and high percentage!!!

THIS QUESTION ISNT THAT BAD actually...

 

[wkawk2416]

if question was asking cholesterol instead of protein, then asnwer would be d.

 

[osimsDDS]

cuz cholesterol is anchored, its not a movable protein in the membrane correct???

 

[wkawk2416]

i don't know about cholestrol being anchored, but it decreases fluidity of PM.

Also, double bond (kinks? kiks? kirks? whatever) , and short phospholipid will also increase the fluidity.

 

[osimsDDS]

but doesnt FRAP have to do with mobility of some substance on the membrane...i dont know if it has to do with the membrane itself being fluid or not...

 

[wkawk2416]

yeah i guess. llol
i don't know why i posted those information.
I was reviewing myself by writing it and summited.

 

[osimsDDS]

yeah i guess. llol
i don't know why i posted those information.
I was reviewing myself by writing it and summited.

what information, about the cholesterol?? or the whole thread?

 

[wkawk2416]

after cholesterol